Transcriptomic profiling between attached and detached SAS cells
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ABSTRACT:
PROVIDER: PRJNA875129 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Cancer cells acquire anchorage-independence to escape anoikis. This study aimed to evaluate the gene expression signature of SAS cells cultured at the standar plate and the low-attachment plate. Total RNAs were isolated from attached and detached SAS cells, and gene expression signatures were examined using gene expression microarray.
2023-01-01 | GSE212367 | GEO
Project description:Gene expression profile between ARID1A knockdown SAS cells and mock SAS cells
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Project description:Transcriptional profiling of SAS cells transfected with pLKO.1-LYRIC shRNA-B expression vector (desinaged as B) and control SAS cells (transfected with pLKO.1 vector, designated as CTL). Goal was to determine the effects of LYRIC knockdown on global SAS cells gene expression.
2016-02-28 | GSE44766 | GEO
Project description:Transcriptional profiling of SAS cells transfected with pLKO.1-LYRIC shRNA-B expression vector (desinaged as B) and control SAS cells (transfected with pLKO.1 vector, designated as CTL). Goal was to determine the effects of LYRIC knockdown on global SAS cells gene expression. Two-condition experiment, SAS cells transfected with pLKO.1-LYRIC shRNA-B expression vector (desinaged as B) v.s. control SAS cells (transfected with pLKO.1 vector, designated as CTL). Biological replicates: 4 control replicates, 4 transfected replicates.
2016-02-28 | E-GEOD-44766 | biostudies-arrayexpress
Project description:Analysis of gene expression profiles of matrix-detached cells with and without expression of ITGB4, in clustering and non-clustering conditions. The experiment tested the hypothesis that the integrin beta 4 (ITGB4) mediates a significant amount of pro-survival signaling in matrix-detached conditions. Expression of ITGB4 in cancer is correlated with poor patient survival and is impliated in increased metastatic spread. Survival in matrix-deprived conditions is essential to metastasis and targeting signaling downstream of the integrin beta 4 may help curtail metasasis.
2019-04-16 | GSE115059 | GEO
Project description:Transcriptional profiling of SAS cells comparing siC-transfected SAS cells with siD-transfected SAS cells. The latter decreased proliferation and migration of SAS cells. Goal was to determine the DDX3-regulated transcripts.
2018-04-17 | GSE113183 | GEO
Project description:The placenta, the complex of fetal membrane (the chorion is attached to the endometrium) and endometrium, is a highly specialized temporary organ responsible for the normal progression of pregnancy in mammals. Therefore, the endometrium was divided into two function regions: chorion-attached (CA) and non-chorion-attached (NCA) region. However, the difference in gene expression profiles between the CA and NCA endometrial tissue of mid-gestation still are unknown. The objective of this study was to reveal gene expressed profiles of the CA and NCA endometrium from the mid-gestational female rabbits, and to screen the differentially expressed genes (DEGs) to imply major physiological events of endometrial at mid-gestation. RNA-seq showed that a total of 12320 genes were co-expressed in the CA and NCA endometrium, and 646 DEGs were identified. Enrichment analysis of the DEGs revealed the top overrepresented gene ontology (GO) term and pathways were related to immune regulation, cellular adhesions. Furthermore, the expression levels of DEGs related to immune regulation of chemokines and their receptors, extracellular matrix (ECM) and Integrin, were compared between the CA and NCA endometrium. Overall, there was hardly difference in the expression levels of immune regulation genes (chemokines and their receptors) between the CA and NCA endometrium, while the concentration of IL-8, IL-6 and IL-1β in the CA endometrium was higher than that in the serum and the NCA endometrium, which suggested immune cytokines were accumulated in the endometrium of maternal-fetal interface. Meanwhile, the expression level of genes related to cellular adhesions (ECM-integrin) in the NCA endometrium was significantly higher than that in the CA endometrium, and high abundance of Integrin β and THBS1 were localized in the luminal epithelium of the NCA endometrium, but not in the CA endometrium. Conclusions: Our study revealed the gene expression profiles of the CA and the NCA endometrium at mid-gestation, and provides an implication of chemokine and ECM-Integrin involved in immune regulation and adhesive barrier at the endometrium.
2020-06-23 | GSE152905 | GEO
Project description:To determine the C1GALT1 regulated gene expression profile in SAS cells
2021-06-28 | GSE116418 | GEO
Project description:Anchorage-independent growth of cancer cells in vitro is correlated to metastasis formation in vivo. Metformin use is associated with decreased breast cancer incidence and currently evaluated in cancer clinical trials. The combined treatment with metformin and 2-deoxy-D-glucose (2DG) in vitro induces detachment of viable MDA-MB-231 breast cancer cells that retain their proliferation capacity. This might be important for cell detachment from primary tumors, but the metabolic changes involved are unknown. We performed LC/MS metabolic profiling on separated attached and detached MDA-MB-231 cells treated with metformin and/or 2DG. High 2DG and metformin plus 2DG altered the metabolic profile similarly to metformin, inferring that metabolic changes are necessary but not sufficient while the specific effects of 2DG are crucial for detachment. Detached cells had higher NADPH levels and lower fatty acids and glutamine levels compared to attached cells, supporting the role of AMPK activation and reductive carboxylation in supporting anchorage-independent survival. Surprisingly, the metabolic profile of detached cells was closer to untreated control cells than attached treated cells, suggesting detachment might help cells adapt to energy stress. Metformin treated cells had higher fatty and amino acid levels with lower purine nucleotide levels, which is relevant for understanding the anticancer mechanisms of metformin.
| S-EPMC8560930 | biostudies-literature
Project description:Metastasis of cancer cells requires detachment from Extra Cellular Matrix (ECM) to seed cancer cells in a distant organ. Hypoxia is prevalent in this matrix detached cancer cells. Studies have established hypoxia as a chromatin modifier, as it transcriptionally controls expression of various histone demethylases (KDMs); therefore, we hypothesized that the presence of hypoxia could modulate the expression of KDMs in matrix detached cancer cells. Our study showed that in matrix detached cancer cells, both hypoxia and one of the hypoxia regulated H3K27me3 histone demethylase, namely KDM6B, was increased. Simultaneously, we found increased expression of stemness-associated genes, namely SOX-2, SOX-9, and CD44, in hypoxic matrix detached cancer cells. We discovered that KDM6B occupies the promoter region of both SOX-2 and CD44 to regulate their expression epigenetically. Targeting KDM6B reduces its occupancy, thereby expressing the stemness-related genes in matrix detached cancer cells. Further, we noticed increased occupancy of HIF1α promoter by KDM6B, suggesting its regulatory role in maintaining hypoxia in matrix detached cancer cells. This observation was further strengthened as we found a significant positive association between KDM6B and HIF1α in various cancer types. Overall, we found that matrix detachment modulates epigenome by inducing KDM6B activity to regulate the expression of stemness-related genes and hypoxia primarily through HIF1α in matrix detached conditions. KDM6B can be developed as a therapeutic target to eliminate matrix detached cancer cells bound for metastatic events
2021-06-11 | GSE176558 | GEO