Project description:The plant signaling molecule auxin controls growth and development through a simple nuclear pathway that regulates the expression of numerous genes. There are however several cellular and physiological responses to auxin that occur within seconds, far too rapid to be mediated by gene expression changes, for which no molecular mechanism has yet been identified. Using a phosphoproteomic strategy in Arabidopsis thaliana roots, an ultra-rapid auxin response system that targets >2000 proteins of which many within 30 seconds was identified.
Project description:Total RNA was extracted from samples including dark-grown WT and tir1 afb123, blue light-grown WT and cry1 cry2 seedlings in continuous conditions for 4.5 days and subjected to high throughput sequencing. Light and auxin co-regulate a variety of developmental processes. This study reveals the molecular basis for light and auxin crosstalk by analyzing gene expression profile under control of both signals. mRNA from dark-grown WT, BL-grown WT, BL-grown BL photoreceptors, and dark-grown auxin receptors was sequenced to analyze the genes regulated by CRY-mediated BL signaling and TIR1/AFBs-mediated auxin signaling.