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THERMO-SENSITIVE BARREN PANICLE (TAP) is required for rice panicle and spikelet development at high temperature

ABSTRACT: THERMO-SENSITIVE BARREN PANICLE (TAP) is required for rice panicle and spikelet development at high temperature

PROVIDER: PRJNA875437 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21377700_1.fastq.gz	Fastqsanger.gz
	SRR21377700_2.fastq.gz	Fastqsanger.gz
	SRR21377701_1.fastq.gz	Fastqsanger.gz
	SRR21377701_2.fastq.gz	Fastqsanger.gz
	SRR21377702_1.fastq.gz	Fastqsanger.gz

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THERMO-SENSITIVE BARREN PANICLE (TAP) is required for rice panicle and spikelet development at high temperature

Project description:We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 genotype (tap mutant and wild type) at two time points and two temperatures.

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Project description:At the transition from vegetative to reproductive growth in rice, a developmental program change occurs, resulting in panicle (rice inflorescence) formation. The initial event of the transition is the change of the shoot apical meristem (SAM) to an inflorescence meristem (IM), accompanied by a rapid increase in the meristem size. Suppression of leaf growth also occurs, resulting in the formation of bracts. The IM generates branch meristems (BMs), indeterminate meristems that reiteratively generate next-order meristems. All meristems eventually acquire a determinate spikelet meristem identity and terminate after producing a floret. ABERRANT PANICLE ORGANIZATION2 (APO2) is the rice ortholog of Arabidopsis (Arabidopsis thaliana) LEAFY (LFY), a plant-specific transcription factor. APO2 is a positive regulator of panicle branch formation. Here, we show that APO2 is also required to increase the meristem size of the IM and suppress bract outgrowth. We identified genes directly and indirectly regulated by APO2 and identified APO2-binding sites by ChIP-seq analysis. These analyses showed that APO2 directly controls known regulators of panicle development, including SQUAMOSA PROMOTER BINDING PROTEIN LIKE14 and NECK LEAF1. Furthermore, we revealed that a set of genes act as downstream regulators of APO2 in controlling meristem cell proliferation at the reproductive transition, bract suppression, and panicle branch formation. Our findings indicate that APO2 acts as a master regulator of rice panicle development by regulating multiple steps in the reproductive transition through directly controlling a set of genes.

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Project description:To gain mechanistic insights on OsMADS2 functions in rice spikelet development, we have employed global expression profilling to identify the genes regulated by OsMADS2. Transgenic rice panicle RNAs were compared with the wild type panicle RNA of similar developmental stage. Genes downregulated in transgenic lines will be those which expression requires functional OsMADS2 whereas upregulated genes will need OsMADS2 for their repression. Experiment Overall Design: Two independent biological RNA pools of dsRNAiOsMADS2 transgenic panicles were compared with a single wild type RNA pool in two different hybridizations. A third hybridization was performed with reciprocally dye-lebeled wild type RNA and one of the mutant RNA.

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2012-11-28 | E-GEOD-38665 | biostudies-arrayexpress

Dynamic profile of gene expression in rice panicle under high temperature

Project description:To comprehend the profile of rice gene expression at reproductive stage under high temperature, Agilent 4×44k rice oligo microarray experiments were carried out using rice panicle of developmental stage 7-9 at 0min, 20min, 1hr, 2hr, 4hr, and 8hr after the treatment of 40 degree centigrade, and the significantly expressed genes mainly involved in transcriptional regulation, transport, cellular homeostasis, and stress response were identified. Among them, the predominant transcription factor gene families were Hsf, NAC, AP2/ERF, WRKY, MYB, and C2H2. KMC analysis discovered the time-dependent gene expression pattern under heat. The results of motif co-occurrence on the promoters of genes from an early up-regulated cluster showed the important roles of GCC box, HSE, ABRE, and CE3, and unraveled the possible cross-talk mechanism during heating. The response model central to ROS combined with transcriptome data indicated the great importance to maintain ROS balance in heat response in rice panicle and the wide existing of cross-talks.

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tap water

Project description:tap water Raw sequence reads

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