Project description:We have compared the gene expression profile of post-natal 1 day and 7 day rat Achilles tendons. Post-natal 1 day and 7 day rat Achilles tendons were collected. Each sample contains at least two individuals. Total RNA was extracted and fragmented biotin-tagged cRNA was hybridized to Rat Genome 230 2.0 Array.
Project description:We used micro-dissection with FACS sorting techniques to isolate renal vesicle single cell types from post natal (P4) kidneys. A subset of these single cell populations is analysed individually via Fluidigm single cell analysis. This analysis will determine the transcriptional profile of each cell type, identify compartment specific transcripts, compartment specific transcript isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database. Kidneys are harvested from Tg(Crym-EGFP)GF82Gsat mice. Single cells are extracted from P4 renal vesicles using micro-dissection with FACS sorting techniques. A subset of these cells is analyzed individually via Fluidigm single cell analysis. The long term goal is to generate a transcriptional atlas of the developing kidney.
Project description:The purpose of this single cell experiment is to compare and characterize at molecular level oesophageal epithelial cells at different points in post-natal development (post-natal day 7 and 28) and at the onset of homeostasis (over p70). With the aim of identifying differences in timepoints showing the key molecular markers of the transition from a rapidly expanding tissue to one that is stable in size and function. Previous data has suggested this transition point occurs around P28 and we hope to see here a similarity in the profile of P28 samples with that of adults. Single cell suspensions of pooled oesophageal epithelial and stroma cells were sorted for EpCam+ and CD45- expression to select for epithelium cells for RNA-sequencing.
Project description:This study aims at giving an insight on gene expression in CCAM. Keywords: disease state analysis A total of 14 microarrays were completed. Samples were divided into four groups: Fetal CCAM samples (n=4), fetal control samples (n=2), post-natal CCAM samples (n=5), post-natal control samples (n=3)
Project description:Although there have been studies conducted on cornea and retina growth and development, postnatal gene expression studies on sclera growth during postnatal growth has not been well characterised. Given that the mouse genome has 85% homology to the human genome and has been completely sequenced, mouse model for the study of ocular growth has advantages over other animal models. Thus, we aimed to study the biology and genetics behind sclera growth during post-natal development in Balb/cJ mice as a means to understand genetic changes that cause scleral growth and development during post-natal eye development The purpose of this study was to identify the genes underlying the development of mouse sclera post-natal growth of the posterior chamber of the eye using microarray