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RNA-Seq based gene expression profiling of mouse Meis1-Meis2 double KO or wild-type atria and ventricles (Embryo).

ABSTRACT: RNA-Seq based gene expression profiling of mouse Meis1-Meis2 double KO or wild-type atria and ventricles (Embryo).

PROVIDER: PRJNA880595 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21570584.fastq.gz	Fastqsanger.gz
	SRR21570585.fastq.gz	Fastqsanger.gz
	SRR21570586.fastq.gz	Fastqsanger.gz
	SRR21570587.fastq.gz	Fastqsanger.gz
	SRR21570588.fastq.gz	Fastqsanger.gz

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RNA-Seq based gene expression profiling of mouse Meis1-Meis2 double KO or wild-type atria and ventricles (Embryo).

Project description:The goal of this study was to compare the gene expression profile, using RNA-seq technology, of mouse Meis1-Meis2 double KO or wild-type fetal.

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RNA-Seq based gene expression profiling of mouse Meis1-Meis2 double KO or wild-type atria and ventricles (Adult).

Project description:The goal of this study was to compare the gene expression profile, using RNA-seq technology, of mouse Meis1-Meis2 double KO or wild-type fetal or adult hearts.

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RNA-Seq based gene expression profiling of mouse Meis1-Meis2 double KO or wild-type atria and ventricles

Project description:RNA-Seq based gene expression profiling of mouse Meis1-Meis2 double KO or wild-type atria and ventricles

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RNA-Seq based gene expression profiling of mouse Meis1-Meis2 double KO or wild-type atria and ventricles (Adult).

Project description:RNA-Seq based gene expression profiling of mouse Meis1-Meis2 double KO or wild-type atria and ventricles (Adult).

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RNA-Seq based gene expression profiling of mouse epicardial-cells with Meis1-Meis2 double KO or wild-type genotypes.

Project description:The goal of this study was to compare the gene expression profile, using RNA-seq technology, of mouse epicardial-cells with Meis1-Meis2 double KO or wild-type genotypes.

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Expression data from normal atria and ventricles

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Developmental gene expression differences between neonatal and adult mouse atria and ventricles

Project description:We study the role of glycosylation in ion channel function. Specfically, we are focusing on how ion channel glycosylation modulates, controls, and impacts cardiac, skeletal muscle, and neuronal electrical activity. We wish to determine differences in gene expression through development and between the atria and ventricles of the mouse heart. Our data indicate differential sialylation directly affects voltage-gated sodium channel function through the developing heart in a chamber-specific manner. We wish to expand our findings to include other ion channels involved in the cardiac action potential, and to eventually create a map of the cardiac conduction system that details the role of differential glycosylation in cardiac excitability. Determining differential expression of the genes that regulate ion channel glycosylation is vital to these goals. We analyzed four sets of pooled RNA to be run in triplicate: one each from neonatal and adult mouse atria and ventricles.

2011-03-02 | E-GEOD-27589 | biostudies-arrayexpress

Identification of gene targets of Meis2

2012-08-03 | GSE34541 | GEO

Identification of gene targets of Meis2

Project description:The homeodomain protein Meis1 is essential for definitive hematopoiesis and vascular patterning in the mouse embryo. Meis2, another member of the same family, shares 82% protein identities with Meis1. Our present study suggested Meis2 exerts two distinguishable effects in differentiating ES cells. First, it increases the numbers of hematopoietic progenitors and extends their persistence in culture. Second, Meis2 skews hematopoietic differentiation by suppressing erythroid while enhancing megakaryocytic progenitor differentiation. To identify the underlying transcriptional bases of these actions, we carried out microarray analysis to compare the various populations of cells developing in ES differentiation cultures in the presence and absence of Meis2 induction. ES cells with dox-inducible Meis2 (A2lox.Meis2) were differentiated as embryoid bodies (EBs) for 6 days before plating on OP9-GFP cell monolayers and cytokines, and treated with (+) or without (-) doxycycline (dox). Cells were purified by cell sorting on day 7 or 8 into various populations based on levels of CD41 expression: GFP-CD41-, GFP-CD41+ (day 7) and GFP-CD41-,GFP-CD41int, and GFP-CD41hi (day 8). Gene expression of these purified populations was determined by microarray analysis.

2012-08-02 | E-GEOD-34541 | biostudies-arrayexpress

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Project description:Pharmacological and gene ablation studies have demonstrated a crucial role of the cardiac natriuretic peptides (NP) hormones ANF and BNP in the maintenance of cardiovascular homeostasis. In addition, hypertension and chronic congestive heart failure are clinical entities that may be regarded as states of relative NP deficiency. Hence the study of the function of the endocrine heart is highly relevant. To identify genes that are related to the endocrine function of the heart we have conducted differential gene expression studies of the rat atria and ventricles using oligonucleotide arrays. Experiment Overall Design: The atrial appendages and the ventricular free walls were obtained from thirteen normal male Sprague Dawley rats. The total RNA was obtained from four pools of atrial and four pools of ventricular tissues. Four biological replicates for each muscle type were generated, i.e. 4 atrial replicates and 4 ventricular replicates.

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