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A novel kinase inhibitor for b-catenin mutant hepatocellular carcinoma [Cut&Run]

ABSTRACT: A novel kinase inhibitor for b-catenin mutant hepatocellular carcinoma [Cut&Run]

PROVIDER: PRJNA881282 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21604065_1.fastq.gz	Fastqsanger.gz
	SRR21604065_2.fastq.gz	Fastqsanger.gz
	SRR21604066_1.fastq.gz	Fastqsanger.gz
	SRR21604066_2.fastq.gz	Fastqsanger.gz
	SRR21604067_1.fastq.gz	Fastqsanger.gz

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CUT&RUN LoV-U of H3K4me3, b-catenin, and IgG in HCT116 cells

Project description:We performed high numbers of replicates of CUT&RUN LoV-U against H3K4me3, β-catenin, and the negative control IgG in human colorectal cancer HCT116 cells over two independent rounds of experiments to discover the complete set of binding events.

2023-07-21 | E-MTAB-13143 | biostudies-arrayexpress

CUT&RUN LoV-U of beta-catenin, TCF/LEF transcription factors and histone modifications in HEK293T and mouse embryonic hindlimb tissue at 11.5 days post coitum

Project description:In our attempts to profile different regulators of the WNT/b-catenin transcriptional complex, CUT&RUN failed to produce consistent binding patterns of the non-DNA-binding b-catenin. We developed a modified CUT&RUN protocol, which we refer to as LoV-U (Low Volume and Urea), that enables the generation of robust and reproducible b-catenin binding profiles. CUT&RUN-LoV-U can profile all classes of chromatin regulators tested, as shown by datasets targeting the TCF/LEF transcription factors and various histone modifications. CUT&RUN-LoV-U uncovers direct WNT/β-catenin target genes in human cells, as well as in ex vivo cells isolated from developing mouse tissue.

2022-07-16 | E-MTAB-11951 | biostudies-arrayexpress

A novel kinase inhibitor for b-catenin mutant hepatocellular carcinoma II

Project description:A novel kinase inhibitor for b-catenin mutant hepatocellular carcinoma II

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A novel kinase inhibitor for b-catenin mutant hepatocellular carcinoma [RNA-seq]

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Project description:Serine-threonine kinase receptor-associated protein (STRAP) is upregulated in breast, colorectal and lung cancers, promoting their growth. We identify the upregulation of STRAP in hepatocellular carcinomas. Elevated STRAP endows tumor cells with growth advantage by reprograming a variety of metabolic processes and signaling pathways critical for hepatocellular carcinoma progression. Especially, enhanced Wnt/β-catenin signaling is likely to be a major effector of its tumor-promoting role.

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CUT&RUN HUDEP1 OE SOX6-Flag

Project description:SOX6 CUT&RUN on HUDEP1 over expressing SOX6-Flag. The experiment is done using and anti Flag Ab to assist the genome wide binding profile of SOX6 in HUDEP1 (Human Umbilical cord blood-Derived Erythroid Progenitor-1).

2023-04-07 | E-MTAB-12800 | biostudies-arrayexpress

CUT&RUN LoV-U of H3K4me3, b-catenin, and IgG in HCT116 cells

Project description:CUT&RUN LoV-U of H3K4me3, b-catenin, and IgG in HCT116 cells

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CUT&RUN experiment at four time points in HEK293T and hESC cells, assessing the time-resolved response of β-catenin genomic occupancy upon Wnt signaling activation

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2022-09-30 | E-MTAB-12077 | biostudies-arrayexpress

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