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ABSTRACT: Sequencing of ovary transcriptome of Exopalaemon carinicauda under saline alkali stress

PROVIDER: PRJNA881755 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR21619473_1.fastq.gz	Fastqsanger.gz
	SRR21619473_2.fastq.gz	Fastqsanger.gz
	SRR21619474_1.fastq.gz	Fastqsanger.gz
	SRR21619474_2.fastq.gz	Fastqsanger.gz
	SRR21619475_1.fastq.gz	Fastqsanger.gz

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Project description:Salt stress, especially saline-alkali stress, has seriously negative effect on citrus production. Ziyang xiangcheng (Citrus junos Sieb.) (Cj) has been reported as a saline-alkali stress and iron deficiency tolerant citrus rootstock. However, the molecular mechanism of its saline-alkali stress tolerance is still not clear. Two citrus rootstocks and one navel orange scion, Cj, Poncirus trifoliate (Poncirus trifoliata (L.) Raf.) (Pt) and ‘Lane Late’ navel orange (Citrus sinensis (L.) Osb.) (LL), were used in this study. The grafted materials Cj+LL and Pt+LL grown in calcareous soil were used to identify genes and pathways responsive to saline-alkali stress using RNA-seq. The seedlings of Cj and Pt grown in the solutions with different gradient pH value were used to perform a supplement experiment. Comprehensively analyzing the data of RNA-seq, physiology and biochemistry, agronomic traits and mineral elements of Cj+LL, Pt+LL, Cj and Pt, several candidate pathways and genes were identified to be highly regulated under saline-alkali stress. Here, we propose citrate is important for the tolerance to iron deficiency and the jasmonate (JA) biosynthesis and signal transduction pathway may play a crucial role in tolerance to saline-alkali stress in citrus by interacting with other plant hormones, calcium signaling, ROS scavenging system and lignin biosynthesis.

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Project description:In this study, an integrative analysis of the gill-specific proteome at 0 h, 12 h and 36 h after alkalinity stress was performed to identify important regulators and pathways involved in alkalinity adaption of Exopalaemon carinicauda. This study reveals the first time-course, gill-specific, combined proteomic profiling associated with alkalinity adaption of E. carinicauda and provides new insights into the mechanisms underlying the molecular response to alkalinity stress in shrimp.

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