Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Bacterial community composition in different color morphs of Diaphorina citri

PROVIDER: PRJNA881837 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21620800_1.fastq.gz	Fastqsanger.gz
	SRR21620800_2.fastq.gz	Fastqsanger.gz
	SRR21620785_1.fastq.gz	Fastqsanger.gz
	SRR21620785_2.fastq.gz	Fastqsanger.gz
	SRR21620786_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

Project description:Infected HLB of different color morphs Diaphorina citri adult

| PRJNA905205 | ENA

Differentially expressed proteins wereassociated withtwo color morphs of Asian citrus psyllid, Diaphorina citri

Project description:Diaphorina citriis a vector of ‘CandidatusLiberibacter asiaticus,’(CLas), associated with citrus greening or Huanglongbing (HLB) disease in citrus. D. citriexhibits two group three different color morph variants, blueand non-blue (includes gray and yellow). Blue morphs have a greater capacity for long-distance flight while has lower efficiency in CLas transmission as compared to non-blue morphswhich influences disease epidemiology. In this study, wecompare the protein profilesof two-color morphsand evaluate the effect of CLas infection on abundance of pre-identified proteins in each color morphs. Our results showed that blue morphs have higher abundance of the immunity-associated proteins, while their abundance were upregulated dramatically in the non-blue morphcompared to blue morphin result of CLas infection. This observation proposes blue morph has higher immunity and CLas trigger the immunity in both morph while in non-blue’s response is late and non-effective.Further, challenging both color morphs byentomopathogenic fungi Beauveria bassiana, were showed significantly lower mortality in blue morph vs non-blue. Also, to test the effect of delayed response in CLas acquisition, we did CLas acquisition assay after priming immunity by feeding heat-killed Liberibacter crescens to both color-morphs. The obtained results were showed that priming immunity in non-blue morph can significantly decrease CLas acquisition as close as the level of CLas acquisition in blue morph. Taking all these in account propose that higher immunity in blue morph suppress CLas acquisition in a way that CLas acts as a pathogenand costly forACP. While in non-blue ACP, CLas triggers the immunity but the delayed immunity is not effective to block CLas from acquisition.

2021-04-26 | PXD017150 | Pride

Project description:The infected Clas of different tissues bacterial community of Diaphorina citri

| PRJNA904527 | ENA

Asian citrus psyllid proteomics: CLas(-) whole adult ACP

2017-02-27 | PXD004454 | Pride

Asian citrus psyllid proteomics: CLas(+) whole nymph ACP

2017-02-27 | PXD004229 | Pride

Asian citrus psyllid proteomics: CLas(+) whole adult ACP

2017-02-27 | PXD004230 | Pride

Asian Citrus Psyllid proteomics: CLas(-) whole ACP nymph

2017-02-27 | PXD004225 | Pride

Asian citrus psyllid proteome: whole insect samples, with or without Candidatus Liberibacter asiaticus

Project description:‘Candidatus Liberibacter asiaticus’ (CLas), the bacterial pathogen associated with citrus greening disease, is transmitted by Diaphorina citri, the Asian citrus psyllid (ACP). We used quantitative mass spectrometry to compare the proteomes of CLas(+) and CLas(-) populations of D. citri. This experiment used adult mixed sex whole insect samples as starting material for protein extraction. DNA extracted from CLas(+) ACP was tested by qPCR to confirm presence of CLas.

2018-10-25 | PXD003096 | Pride

Comparative proteomic analysis of hemolymph from uninfected and Candidatus Liberibacter asiaticus-infected Diaphorina citri

Project description:Hemolymph was characterized from Diaphorina citri adults infected with the phytopathogen, Candidatus Liberibacter asiaticus (CLas) and compared with that from uninfected psyllids. This study identified 5531 and 3220 peptides within infected and uninfected hemolymph using nano LC-MS/MS. A reduced number of proteins were detected for D. citri and all known endosymbionts within infected hemolymph as compared to uninfected hemolymph. A large number of immune defense proteins were absent from D. citri hemolymph; however, a single recognition protein (PGRP), two serine protease inhibitors, three prophenoloxidase (proPO) enzymes, and a single serine protease in an uninfected D. citri were detected. The hemolymph is nearly devoid of nutrient storage proteins. This is the first proteomic analysis of D. citri hemolymph that also analyzes the components contributed by all of the endosymbionts. By comparing the contribution of each endosymbiont (CCR, CPA,WB) in the presence and absence of CLas infection, this study provides initial insights regarding the hemolymph response to microbial community shifts associated with D. citri infection status. Our data also present potential protein targets for analysis and disruption of CLas transmission that may facilitate management of huanglongbing (HLB) caused by CLas in citrus.

2017-01-18 | PXD005404 | Pride

Project description:A Transcriptomic analysis of body-color polyphenism in Diaphorina citri

| PRJNA939099 | ENA