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Whole-transcriptome analyses of sheep embryonic testicular cells infected with bluetongue virus

ABSTRACT: Whole-transcriptome analyses of sheep embryonic testicular cells infected with bluetongue virus

PROVIDER: PRJNA882007 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21622800.fastq.gz	Fastqsanger.gz
	SRR21622801.fastq.gz	Fastqsanger.gz
	SRR21622802.fastq.gz	Fastqsanger.gz
	SRR21622803.fastq.gz	Fastqsanger.gz
	SRR21622804.fastq.gz	Fastqsanger.gz

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Whole-transcriptome analyses of sheep embryonic testicular cells infected with bluetongue virus [miRNA-Seq]

Project description:To comprehensively understand the characterization of bluetongue virus (BTV)-host interactome, and BTV infection and pathogenic mechanisms, RNA-seq was performed with BTV serotype 1 Y863 strain-infected and mock-infected sheep embryonic testicular cells (OA3.Ts) at 24 hours post-infection.

2022-11-28 | GSE213638 | GEO

Whole-transcriptome analyses of sheep embryonic testicular cells infected with bluetongue virus [RNA-Seq]

2022-11-28 | GSE213637 | GEO

Whole-transcriptome analyses of sheep embryonic testicular cells infected with bluetongue virus [RNA-Seq]

Project description:Whole-transcriptome analyses of sheep embryonic testicular cells infected with bluetongue virus [RNA-Seq]

| PRJNA882010 | ENA

Whole-transcriptome analyses of sheep embryonic testicular cells infected with bluetongue virus [miRNA-Seq]

Project description:Whole-transcriptome analyses of sheep embryonic testicular cells infected with bluetongue virus [miRNA-Seq]

| PRJNA882011 | ENA

Whole-transcriptome analyses of sheep embryonic testicular cells infected with the bluetongue virus.

Project description:Introductionbluetongue virus (BTV) infection triggers dramatic and complex changes in the host's transcriptional profile to favor its own survival and reproduction. However, there is no whole-transcriptome study of susceptible animal cells with BTV infection, which impedes the in-depth and systematical understanding of the comprehensive characterization of BTV-host interactome, as well as BTV infection and pathogenic mechanisms.Methodsto systematically understand these changes, we performed whole-transcriptome sequencing in BTV serotype 1 (BTV-1)-infected and mock-infected sheep embryonic testicular cells, and subsequently conducted bioinformatics differential analyses.Resultsthere were 1504 differentially expressed mRNAs, 78 differentially expressed microRNAs, 872 differentially expressed long non-coding RNAs, and 59 differentially expressed circular RNAs identified in total. Annotation from the Gene Ontology, enrichment from the Kyoto Encyclopedia of Genes and Genomes, and construction of competing endogenous RNA networks revealed differentially expressed RNAs primarily related to virus-sensing and signaling transduction pathways, antiviral and immune responses, inflammation, and development and metabolism related pathways. Furthermore, a protein-protein interaction network analysis found that BTV may contribute to abnormal spermatogenesis by reducing steroid biosynthesis. Finally, real-time quantitative PCR and western blotting results showed that the expression trends of differentially expressed RNAs were consistent with the whole-transcriptome sequencing data.Discussionthis study provides more insights of comprehensive characterization of BTV-host interactome, and BTV infection and pathogenic mechanisms.

| S-EPMC9751015 | biostudies-literature

Whole-transcriptome analyses of sheep embryonic testicular cells infected with bluetongue virus

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-11-29 | GSE213640 | GEO

Dendritic cell subtypes from lymph nodes and blood show contrasted gene expression programs upon Bluetongue Virus infection

2013-07-09 | GSE48450 | GEO

Proteomic analysis of sheep primary testicular cells infected with bluetongue virus.

Project description:Bluetongue virus (BTV) causes a non-contagious, arthropod-transmitted disease in wild and domestic ruminants, such as sheep. In this study, we used iTRAQ labeling coupled with LC-MS/MS for quantitative identification of differentially expressed proteins in BTV-infected sheep testicular (ST) cells. Relative quantitative data were obtained for 4455 proteins in BTV- and mock-infected ST cells, among which 101 and 479 proteins were differentially expressed at 24 and 48 h post-infection, respectively, indicating further proteomic changes during the later stages of infection. Ten corresponding genes of differentially expressed proteins were validated via real-time RT-PCR. Expression levels of three representative proteins, eIF4a1, STAT1 and HSP27, were further confirmed via western blot analysis. Bioinformatics analysis disclosed that the differentially expressed proteins are primarily involved in biological processes related to innate immune response, signal transduction, nucleocytoplasmic transport, transcription and apoptosis. Several upregulated proteins were associated with the RIG-I-like receptor signaling pathway and endocytosis. To our knowledge, this study represents the first attempt to investigate proteome-wide dysregulation in BTV-infected cells with the aid of quantitative proteomics. Our collective results not only enhance understanding of the host response to BTV infection but also highlight multiple potential targets for the development of antiviral agents.

| S-EPMC7168089 | biostudies-literature

Dendritic cell subtypes from lymph nodes and blood show contrasted gene expression programs upon Bluetongue Virus infection

Project description:The gene expression in conventional and plasmacytoid dendritic cells (cDC and pDC respectively) during Bluetongue virus (BTV) infection in sheep depends on the lymphoid compartment and suggest that these cell types have a role in the physiopathology We analyzed the gene expression in lymph node pDC and cDC from 3 control and 3 BTV infected sheep at day 6 post infection, in blood pDC from 2 control and 2 BTV infected sheep at day 6 post infection, and in spleen cDC from 3 control and 2 BTV infected sheep at day 6 post infection.

2013-07-09 | E-GEOD-48450 | biostudies-arrayexpress

Phosphoproteomic analysis of Bluetongue virus infection

Project description:Bluetongue virus causes infections in wild and domesticated ruminants, with the potential for causing significant morbidity, mortality and economic harm in both the developing and developed world. While vaccines have been developed, no treatment for acute infections exists. In this regard, a possible approach is to determine host-cell factors utilised by Bluetongue virus. We thus proceeded to characterize the phosphoproteome of BTV infected HeLa cells to elucidate the intracellular signalling pathways and identify critical host factors activated during Bluetongue virus infection.

2017-08-29 | PXD005550 | Pride

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