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Single-Cell RNA-Seq Analysis Reveals Macrophage Involved in Pathogenesis of Human sporadic type A aortic dissection

ABSTRACT: Single-Cell RNA-Seq Analysis Reveals Macrophage Involved in Pathogenesis of Human sporadic type A aortic dissection

PROVIDER: PRJNA882355 | ENA |

REPOSITORIES: ENA

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Single-Cell RNA-Seq Analysis Reveals Macrophage Involved in Pathogenesis of Human sporadic type A aortic dissection

Project description:Macrophages play an important role in the progression of sporadic acute type A aortic dissection (ATAAD). The aim of this study was to characterize the cellular heterogeneity of macrophages in AD tissues by scRNA-seq. Ascending aortic wall tissue from 6 ATAAD patients and 3 heart transplant donors were assessed by scRNA-seq, then analyzed and validated by various bioinformatics algo-rithms and histopathology experiments. The results revealed the proportion of macrophages in AD tissues (24.51%) was significantly higher than that in normal tissues (13.69%). Among the 6 macro-phage subclusters, pro-inflammatory macrophages accounted for 14.96% in the AD group, and 0.18% in the normal group. Chemokine and inflammation related genes (CCL2, CCL20, S100A8, and S100A9) were expressed more intensively in macrophages in AD tissue than that in the normal tissues. Ad-ditionally, intercellular communication analysis and transcription factor analysis indicated activation of inflammation and degradation of the extracellular matrix in AD tissues. Finally, immunohisto-chemistry, immunofluorescence and western blot experiments confirmed the overexpression of macrophage marker genes (CD68 and CD163) and matrix metalloproteinases (MMP9 and MMP2) in AD tissues. Collectively, our study provides a preliminary evaluation of the role of macrophages in AD and potentially aid in the development of therapeutic options in the future.

2022-09-23 | GSE213740 | GEO

The role of macrophage Socs3 in the pathogenesis of aortic dissection

Project description:The role of macrophage Socs3 in the pathogenesis of aortic dissection

| PRJNA420217 | ENA

The role of macrophage Socs3 in the pathogenesis of aortic dissection

Project description:Background—Aortic dissection (AD) is a life-threatening medical emergency caused by the abrupt destruction of the intimomedial layer of the aortic walls. As previous studies have reported the involvement of proinflammatory cytokine IL-6 in AD pathogenesis, we investigated the role of Stat3 signaling, a downstream pathway of IL-6 in macrophages in pathogenesis of AD. Methods and Results—We characterized the pathological and molecular events triggered by aortic stress, which can lead to AD. Aortic stress on the suprarenal aorta due to infrarenal aorta stiffening and angiotensin II infusion for 1 week caused focal medial rupture at the branching point of the celiac trunk and superior mesenteric artery. This focal medial rupture in 6 weeks in wild type (WT) mice but progressed to AD in mice with macrophage-specific deletion of Socs3 gene (mSocs3-KO). mSocs3-KO mice showed premature activation of cell proliferation, an inflammatory response, and skewed differentiation of macrophages toward the tissue-destructive phenotype. Concomitantly, they showed aberrant phenotypic modulation of SMCs and TGFβ signaling, which are likely to participate in tissue repair. Human AD samples revealed STAT3 activation in adventitial macrophages adjacent to the site of tissue destruction. Conclusions—These findings suggest that AD development is preceded by focal medial rupture, in which macrophage Socs3 maintains proper inflammatory response and differentiation of SMCs, thus promoting fibrotic healing to prevent tissue destruction and AD development. Understanding the sequence of the pathological and molecular events preceding AD development will help predict and prevent AD development and progression.

2017-11-30 | GSE107479 | GEO

aortic dissection molecular markers using iTRAQ combined with Label Free techniques

Project description:To screen serum molecular markers systematically amid aortic dissection and acute coronary syndrome, preliminary identify the pathogenesis of acute aortic dissection.

2022-03-25 | PXD032857 |

Expression data from control, aortic aneurysm, and aortic dissection cells

Project description:Aneurysmatic and dissection cells show a specific alteration of gene expression, which allow a disease specific distinction. We used microarrays to analyse the cellular gene expression of controls, thoracic aortic aneurysm, and aortic dissection.

2022-12-02 | GSE219204 | GEO

Epigenome analysis of ascending aorta from normal, aortic dissection and bicuspid aortic valve patients

Project description:Genome wide DNA methylation profiling of ascending aorta tissue samples from normal, aortic dissection and bicuspid aortic valve patients with aortic dilation. The Illumina Infinium 450k Human DNA methylation Beadchip was used to obtain DNA methylation profiles across more than 450,000 CpGs in ascending aorta samples. Samples included 6 normal donors, 12 patients with aortic dissection and 6 patients with bicuspid aortic valve and dilated aorta.

2019-07-11 | GSE84274 | GEO

Expression data from aortic dissection patients and the healthy controls

Project description:The mechanisms of acute aortic dissection are not well understood. Transcriptomic strategy has been proven to be an effective way to find the potential mechanisms and also the reliable biomarkers for a specific disease. The whole-genome expression profiling was assayed in a panel of aortic tissues from 4 male acute aortic dissection patients and 4 male healthy controls.

2021-12-12 | GSE190635 | GEO

Single Cell RNA Sequence Reveals a Distinct Immune Landscape in Acute Aortic Dissection.

Project description:We investigated the relationship between a developemnt of aortic dissection and inflammatory cells. Il-1b high expression macrophage contributed to developing an aortic dissection.

2024-03-01 | GSE224559 | GEO

Genome-wide analysis of gene expression of ascending aorta from patients with acute Stanford type A aortic dissection

Project description:Differentially expressed genes were identified by comparing the gene expression profiling of dissected ascending aorta with that of control. Results provide important information to indicate pathogenesis of aortic dissection.

2014-12-31 | GSE52093 | GEO

Analysis of aortic gene expression in mice with aortic dissection treated with colchicine

Project description:Colchicine is an alkaloid found in the plant Colchicum, which is currently used for the treatment of acute gout and familial Mediterranean fever, and is being considered for the treatment of a variety of cardiovascular diseases such as pericarditis, atrial fibrillation, and coronary syndromes due to its significant anti-inflammatory effects. Aortic dissection is a cardiovascular disease characterised by a tear in the intima and congestion of the vessel wall leading to vessel wall delamination, with a rapid onset and high lethality. This study investigated the role of colchicine in BAPN-induced aortic dissection in mice. Gavage administration of colchicine along with BAPN modelling for three weeks showed a significant decrease in morbidity and mortality, indicating that colchicine can inhibit the occurrence of aortic dissection in mice.

2024-10-25 | GSE279545 | GEO

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