Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of tet-inducible expression of individual MAP3Ks (COT, MEKK1, MEKK2, MEKK3, ASK1, ASK3, ARAF, RAF1, BRAF, NIK, ZAK1, MLK3, MLK1) in MCF10A mammary epithelial cells.

ABSTRACT: Effect of tet-inducible expression of individual MAP3Ks (COT, MEKK1, MEKK2, MEKK3, ASK1, ASK3, ARAF, RAF1, BRAF, NIK, ZAK1, MLK3, MLK1) in MCF10A mammary epithelial cells.

PROVIDER: PRJNA882846 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21660869_1.fastq.gz	Fastqsanger.gz
	SRR21660869_2.fastq.gz	Fastqsanger.gz
	SRR21660870_1.fastq.gz	Fastqsanger.gz
	SRR21660870_2.fastq.gz	Fastqsanger.gz
	SRR21660871_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 60

Similar Datasets

Gene expression profiles of ASK1-deficient adipose tissues

Project description:To elucidate the potential function of ASK1 in adipose tissues, microarray analysis was performed using iBAT and eWAT. Tissue samples were collected from 10-week-old male mice, and RNA samples derived from three individuals were pooled to analyze. These data provide novel insights into the physiological functions of ASK1. To elucidate the potential function of ASK1 in adipose tissues, microarray analysis was performed using iBAT and eWAT.

2016-01-08 | E-GEOD-76660 | biostudies-arrayexpress

Endothelial Raf1 activation regulates arterial morphogenesis

Project description:Analyze effect of Raf1 S259A on gene expression in HUVEC Total RNA obtained from HUVEC infected with empty lentiviral vector pLVX-IRES-puro (control) or that expressing human RAF1 WT (WT) or RAF1 S259A (S259A).

2012-12-18 | E-GEOD-42964 | biostudies-arrayexpress

NIK expression stimulated G6PD phosphorylation

Project description:Our findings establish NIK as a pivotal regulator of T cell metabolism in anti-tumor immunity and highlight a posttranslational mechanism of metabolic regulation involving the G6PD-NADPH redox system. CoIP assays revealed a strong physical interaction between NIK and G6PD in both T cells and transiently transfected 293 cells, suggesting G6PD to be a direct target of NIK. Using a phosphoprotein gel analysis approach, we demonstrated that NIK expression stimulated G6PD phosphorylation. To further study the mechanism, we performed mass spectrometry identify phosphorylation sites of G6PD stimulated by NIK

2020-10-05 | PXD020943 | Pride

Expression data from inflorescence (unopened flower buds) of Arabidopsis thaliana - comparison between Ler and ask1 mutant

Project description:ARABIDOPSIS SKP1-LIKE1 (ASK1) protein is involved in regulating flower development. We have compared ask1 mutant floral transcriptome with wild-type Ler to identify the role of ASK1-containing E3 ubiquitin ligases in regulating flower transcriptome. In this dataset, we include the expression data obtained from Arabidopsis thaliana Ler and ask1 mutant flower buds. We identified 42 genes and 74 genes that are down-regulated and up-regulated, respectively. Totally 5 samples were analyzed: 3 samples of ask1 and 2 samples of Ler. The average values were compared between ask1 and Ler.

2013-02-01 | E-GEOD-42841 | biostudies-arrayexpress

Effect of tet-inducible expression of individual MAP3Ks (COT, MEKK1, MEKK2, MEKK3, ASK1, ASK3, ARAF, RAF1, BRAF, NIK, ZAK1, MLK3, MLK1) in MCF10A mammary epithelial cells.

Project description:To investigate the cooperative function ERK, JNK and p38 signaling in cell behavior decisions, we established MCF10A cell lines in which each target gene has been overexpressed by stable integration of a TRE3G (TET) inducible promoter driver the expression of each target gene. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 independent repliatse at 18 hours post induction.

2022-09-26 | GSE213882 | GEO

Gene expression profiles of ASK1-deficient adipose tissues

2016-01-08 | GSE76660 | GEO

Gene expression data from MCF7 cells overexpressing RAF1

Project description:In this study we performed gene transcription analysis of parental MCF7 cells and MCF7 engineered to overexpress oncoprotein RAF1. We demonstrate that overexpression of RAF1 leads to development of ivasive phenotype.

2017-08-31 | GSE100360 | GEO

OTUD1 aggravates pathological cardiac hypertrophy through activation of PGAM5-ASK1 signaling pathways

Project description:Deubiquitinating enzymes have gained more and more attention in the field of pathological cardiac hypertrophy. In this study, we explored the role of a deubiquitinase, OTUD1, in the transverse aortic constriction (TAC) induced cardiac hypertrophy. We found the upregulation of OTUD1 in heart tissues of TAC mice. OTUD1 overexpression promoted cardiac hypertrophy, cardiac fibrosis and apoptosis. Conversely, OTUD1 depletion alleviated these pathological changes both in vivo and in vitro. Mechanistically, ASK1 was identified as one substrate of OTUD1 using co-immunoprecipitation followed with LC-MS/MS. Interestingly, OTUD1 didn’t deubiquitinate ASK1, but increased the phosphorylation level of ASK1 during the process of cardiac hypertrophy. We found that PGAM5, the upper stream regulator of ASK1, was stabilized by OTUD1 in a K63 ubiquitin chain dependent way, which reminded us OTUD1 increased the phosphorylation level of ASK1 by deubiquitinating PGAM5. This study identified the OTUD1-ASK1 axis as a potential therapeutic target for pathological cardiac hypertrophy.

2023-09-05 | GSE241809 | GEO

Endothelial Raf1 activation regulates arterial morphogenesis

Project description:Analyze effect of Raf1 S259A on gene expression in HUVEC

2012-12-18 | GSE42964 | GEO

Comparasion between MCF7-NIK overexpressing cells, MCF7-control cells and MCF7-NIK depleted cells

Project description:Analysis of differentially expressed genes in MCF7 cancer cells transfected with an expression vector containing the ORF of NIK, a shRNA of NIK and a control shRNA. Transient transfections were performed in triplicates . We use micorarrays to elucidate the machanisms by which NIK regulates stem cells biology

2015-11-30 | GSE63382 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data