Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

EC_tagging pulse-chase to measure mRNA decay in Drosophila larval neuroblasts and neurons

ABSTRACT: EC_tagging pulse-chase to measure mRNA decay in Drosophila larval neuroblasts and neurons

PROVIDER: PRJNA883697 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21684949.fastq.gz	Fastqsanger.gz
	SRR21684950.fastq.gz	Fastqsanger.gz
	SRR21684951.fastq.gz	Fastqsanger.gz
	SRR21684952.fastq.gz	Fastqsanger.gz
	SRR21684953.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

EC_tagging pulse-chase to measure mRNA decay in Drosophila larval neuroblasts and neurons

Project description:Nascent RNA was tagged with EU, via EC feeding, in targeted cells (neuroblasts or neurons) then purified after the initial pulse labeling or after a chase using media containing excess unmodified uridine

2022-09-26 | GSE214062 | GEO

Thiouracil Pulse-chase, Tachyzoites and Bradyzoites mRNA stability data

Project description:Pulse chase measurements using thiouracil (DTU) labeling via UPRT and chasing with uracil Data from tachyzoites is labeled "DTU Pulse Chase". Two independent pulse chase experiments were performed in tachyzoites, pulse chase 1 and 2. Duplicate arrays at each timepoint were performed for pulse chase 2 (2 a and b). Data from bradyzoites are labeled "DTU Bradyzoite Pulse Chase". Two independent pulse chase experiments were performed in bradyzoites and a single set of arrays were performed for each experiment. Just one chase timepoint was used in the bradyzoite experiments, the 2 hour chase. An RNA stablity experiment design type examines stability and/or decay of RNA transcripts. Keywords: RNA_stability_design

2005-07-16 | GSE2947 | GEO

Thiouracil Pulse-chase, Tachyzoites and Bradyzoites mRNA stability data

2005-07-16 | E-GEOD-2947 | biostudies-arrayexpress

Genomewide measurements of whole embryo mRNA decay in Drosophila

Project description:whole embryo (all tissues) measurement of mRNA decay by 4-thiouridine pulse-chase These TU-Decay microarrays analyze mRNA levels at three timepoints: a one hour pulse, one hour chase, and three hour chase. Measurements with or without transcription inhibition by actinomycin D (ActD) were compared.

2015-03-31 | E-GEOD-67435 | biostudies-arrayexpress

Genomewide measurements of whole embryo mRNA decay in Drosophila

Project description:whole embryo (all tissues) measurement of mRNA decay by 4-thiouridine pulse-chase

2015-03-31 | GSE67435 | GEO

Genomewide measurements of neural-specific mRNA decay in Drosophila embryos

Project description:Neural-specific mRNA decay measurements by TU-Decay technique in control and Pumilio knockdown embryos These TU-Decay microarrays analyze mRNA levels at three timepoints: a one hour pulse, one hour chase, and three hour chase. Neural-specific RNA purification was achieved using prospero-GAL4 driving UAS-T.g.UPRT. Pumilio knockdown in the nervous system was acheived using UAS-Pum(RNAi) driven by prospero-Gal4.

2015-04-02 | E-GEOD-67512 | biostudies-arrayexpress

Transcription profiling of Toxoplasma gondii thiouracil pulse-chase, tachyzoites and bradyzoites mRNA stability data

2005-07-07 | E-SMDB-2949 | biostudies-arrayexpress

Azidonucleoside-incorporated RNA sequencing for RNA stability analysis in E.coli.

Project description:To study RNA degradation by RNA-seq, global inhibition of transcription has to be performed in order to measure RNA decay with no interference from RNA sysnthesis. However, transcription inhibitors are generally toxic, can affect abundance many transcripts, and cause delay in RNA decay by residual RNA synthesis. We envisioned that AIR-seq, in conjunction with the pulse-chase labeling, would enable genome-wide analysis of RNA degradation in E. coli with no need of transcription inhibition.

2020-11-01 | GSE144941 | GEO

Cell-specific analysis of mRNA synthesis and decay

Project description:This SuperSeries is composed of the following subset Series: GSE2946: Synthesis vs. Abundance, Bradyzoite Development GSE2947: Thiouracil Pulse-chase, Tachyzoites and Bradyzoites mRNA stability data GSE2948: UPRT Transgenic cells, Human Arrays Abstract: Standard microarrays measure mRNA abundance, not mRNA synthesis, and therefore cannot identify the mechanisms that regulate gene expression. We have developed a method to overcome this limitation by using the salvage enzyme uracil phosphoribosyltransferase (UPRT) from the protozoan Toxoplasma gondii. T. gondii UPRT has been well characterized because of its application in monitoring parasite growth: mammals lack this enzyme activity and thus only the parasite incorporates (3)H-uracil into its nucleic acids. In this study we used RNA labeling by UPRT to determine the roles of mRNA synthesis and decay in the control of gene expression during T. gondii asexual development. We also used this approach to specifically label parasite RNA during a mouse infection and to incorporate thio-substituted uridines into the RNA of human cells engineered to express T. gondii UPRT, indicating that engineered UPRT expression will allow cell-specific analysis of gene expression in organisms other than T. gondii. Refer to individual Series

2006-03-07 | E-GEOD-4401 | biostudies-arrayexpress

Pulse-chase SILAC analysis of mitochondrial complex assembly

Project description:Pulse (P) - Chase (C) SILAC analysis of mitochondrial complex assembly Bogenhagen et al (2018) Cell Reports Pulse-chase times in hours (P#C#)

2020-01-03 | MSV000084750 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data