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Single-cell transcriptomic analysis of human and mouse oligodendrocyte precursor cells

ABSTRACT: Single-cell transcriptomic analysis of human and mouse oligodendrocyte precursor cells

PROVIDER: PRJNA883743 | ENA |

REPOSITORIES: ENA

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Single-cell transcriptomic analysis of human and mouse oligodendrocyte precursor cells

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Investigation of Oligodendrocyte Precursor Cell Differentiation by Quantitative Proteomics

Project description:Oligodendrocytes, the myelinating cells of the central nervous system, are essential for correct brain function. They originate from oligodendrocyte precursor cells (OPCs) through a differentiation process which is only incompletely understood and impaired in a variety of demyelinating diseases. Better knowledge of this differentiation holds the promise to develop novel therapies for these disorders. We investigated the differentiation of rat oligodendrocyte precursor cells to oligodendrocytes in vitro. After confirmation of differentiation by immunohistochemical analysis using cell type-specific marker proteins, we conducted a quantitative proteomics study using tandem mass tags (TMT). We investigated four time points of differentiation covering early, intermediate, and late stages. Data analysis was perfomed using Mascot and Maxquant.

2019-06-21 | PXD012544 | Pride

Single-Cell Transcriptomic Analysis Reveals Molecular Diversity of Human Oligodendrocyte Progenitor Cells

Project description:We performed single-cell-transcriptomic-analysis of developing human stem cell-derived oligodendrocyte-precursor-cells (hOPCs). We studied both a genome engineered hESC-reporter lines, containing an Identification-and-Purification tag expressed under control of the endogenous, OPC-specific, PDGFRa promoter, and an unmodified iPSC line, and performed time-course single-cell-RNA-sequencing of purified hOPCs. Our analysis uncovered transcriptional heterogeneity of PDGFRa+ hOPCs and identified regulatory genes and networks that control their differentiation and myelination competence. Pseudotime trajectory analysis suggested distinct trajectories for the development of oligodendrocytes vs astrocytes from the hOPCs. We also identified novel transcription factors and other genes that developing hOPCs potentially use to choose between oligodendrocyte vs astrocyte lineages. In addition, pathway enrichment analysis followed by pharmacological intervention of those pathways confirm that mTOR and cholesterol biosynthesis signaling pathways are involved in maturation of oligodendrocytes from hOPCs.

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Project description:Oligodendrocytes are generated from oligodendrocyte precursor cells (OPCs). Chd7 is ATP-dependent chromatin-remodeling enzyme. We performed microarray analysis to examine changes in gene expression between control and Chd7 knockdown OPCs. Results provide insight into the function of Chd7 in oligodendrocyte development.

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Project description:The susceptibility to neurological and psychiatric disorders reveals sex-specific differences. Several studies have reported sex-related differences in the structure and function of human brains. Oligodendrocyte precursor cells (OPCs) regulate the neuronal system in various ways and play crucial roles in brain homeostasis besides their well-known role as a reservoir for mature oligodendrocytes. Here, we revealed transcriptomic differences in OPCs isolated from male and female neonatal rat brains.

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Reactive human iPSC astrocytes suppress oligodendrocyte precursor cell differentiation

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2017-12-02 | GSE107582 | GEO

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