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ABSTRACT: transcriptome analysis of litchi

PROVIDER: PRJNA883943 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21707713_1.fastq.gz	Fastqsanger.gz
	SRR21707713_2.fastq.gz	Fastqsanger.gz
	SRR21707714_1.fastq.gz	Fastqsanger.gz
	SRR21707714_2.fastq.gz	Fastqsanger.gz
	SRR21707715_1.fastq.gz	Fastqsanger.gz

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MicroRNAs and targets in senescent fruit of litchi during ambient storage and post cold storage shelf-life.

Project description:Background: Litchi has high commercial value for its bright color and rich nutrients. However, it deteriorates with the pericarp turning brown within 1-2 days after harvest. The factors that mediate litchi fruit senescence are complicated. MicroRNAs act as negative regulators involving in almost every physiological process. To understand the mechanism of litchi fruit senescence and pericarp browning from miRNA level, five small RNA libraries and a degradome library from the pericarp of litchi fruit stored at ambient and post cold shelf-life were sequenced. Results: By aligning the sRNA reads onto litchi unigene assembly, 296 miRNAs belonging to 49 known miRNA families were first identified from litchi. In addition, eleven litchi-specific miRNAs were identified. Among these, 167 known miRNAs were identified to cleave 197 targets, and three litchi-specific miRNAs were found to have five targets. Through combined analysis of stem-loop quantitative real-time polymerase chain reaction (qRT-PCR) and transcriptome profiling, 14 miRNA-target pairs were found to be actively involved in litchi fruit senescence-related processes, including energy regulation, anthocyanin metabolism, hormone signaling, and pathogen-infection defense. Conclusions: A network of miRNA-target that regulates litchi fruit senescence has been proposed, revealing the miRNA-mediated regulation in senescent litchi fruit. This will aid to develop new strategies to postpone the senescence of litchi fruit and other horticultural products.

2017-02-13 | GSE63658 | GEO

Transcriptome analysis of the litchi fruit borer (Conopomorpha sinensis)

Project description:Purpose: transcriptome sequencing of Conopomorpha sinensis Methods: high-through Illumina HiSeqTM 2000 Results:66017 transcripts,35383 unigenes Conclusions:This study provided valuable transcriptome data for the litchi fruit borer, which was the first fundamental genomic basis for exploiting gene resources from the litchi fruit borer

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Transcriptome analysis of Litchi chinensis under inoculated with P.litchii treatments

Project description:To investigate the potential mechanism of disease resistance in Litchi, a genome-wide transcriptomic analysis was carried out using 'Guiwei' and 'Yurong1' Litchi under inoculated with P.litchii treatments.

2022-08-01 | GSE201243 | GEO

Transcriptomics‐based analysis of the causes of “sugar receding” in ‘Feizixiao’ litchi (Litchi chinensis Sonn.) pulp

Project description:Transcriptome sequencing of ‘Feizixiao' litchi pulp at 35, 63 and 69 days after anthesis

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Litchi chinensis

Project description:transcriptome analysis of litchi

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Project description:Transcriptome analysis of litchi

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Transcriptome and proteome analysis revealed the preservation mechanism of litchi.

Project description:Transcriptome and proteome were used to conjoint analysis the preservation mechanism of the citric acid combined with L-cysteine on post-harvested litchi fruit.

2024-08-12 | PXD054833 |

Coupling of microRNA-directed phasiRNA generation with alternative splicing in small RNA-mediated gene silencing

Project description:Purpose:We aimed to characterize genome-widely the miRNA population and phasiRNA-generating genes/loci in litchi (Litchi chinensis). Multiple high throughput sequencing strategies, including sRNA sequencing, parallel analysis of RNA ends (PARE) sequencing, and strand-specific RNA-seq, were applied in combination with thorough bioinformatics analyses using a variety of computational methods.

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Project description:transcriptome analysis of litchi pericarp

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Analysis of gene expression signatures for Litchi knockout during motor neuron differentiation

Project description:We identified Litchi (Gm2990) as a novel regulator for orchestrating motor neuron (MN) maturation. To study the function of Lithci during MN differentiation, we knocked out Litchi in mouse ESCs and differentiated into MN.

2024-03-11 | GSE248380 | GEO

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