Project description:Ginsenosides are a class of natural product triterpene saponins and almost exclusively in the plant genus Panax which has a long history of use as dietary supplements. Pharmacological research demonstrated that ginsenosides have multiple bioactivities. Ginsenoside is produced at high levels within Panax japonicus, and we have performed Lable-free quantitaion analysis of multiple tissues from this species in order to investigate the biosynthetic genes required for producing ginsenoside.
Project description:In this study, we have performed Illumina based RNA sequencing to characterize the transcriptome and expression profiles of genes expressed in 5 tissues of P. japonicus. RNA sequencing and de novo transcriptome assembly for P. japonicus resulted in a total of 135,235 unigenes with 78,794 (58.24%) unigenes being annotated using NCBI-nr database. Transcriptome profile and GO enrichment analysis for 5 tissues of P. japonicus showed that although each tissue was characterized by several unique unigenes with leaf showing the most unique unigenes among all, overall processes were evenly conserved across all tissues. Examination of 5 tissues of Panax japonicus
Project description:Spatial Protein Expression of Panax Ginseng by In-depth Proteomic Analysis
for Ginsenoside Biosynthesis and Transportation.
2732 proteins and 3608 proteins were identified from ginseng root and cauline leaf, respectively, which was the largest data set reported so far.
Project description:In this study, we have performed Illumina based RNA sequencing to characterize the transcriptome and expression profiles of genes expressed in 5 tissues of P. japonicus. RNA sequencing and de novo transcriptome assembly for P. japonicus resulted in a total of 135,235 unigenes with 78,794 (58.24%) unigenes being annotated using NCBI-nr database. Transcriptome profile and GO enrichment analysis for 5 tissues of P. japonicus showed that although each tissue was characterized by several unique unigenes with leaf showing the most unique unigenes among all, overall processes were evenly conserved across all tissues.
