Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bulk RNAseq analysis of Ecsit-deficient skeletal progenitors

ABSTRACT: Bulk RNAseq analysis of Ecsit-deficient skeletal progenitors

PROVIDER: PRJNA885439 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21753509.fastq.gz	Fastqsanger.gz
	SRR21753510.fastq.gz	Fastqsanger.gz
	SRR21753511.fastq.gz	Fastqsanger.gz
	SRR21753512.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Bulk RNAseq analysis of Ecsit-deficient skeletal progenitors

Project description:Whole transcriptome analysis showing differential gene expression in Ecsit sufficient vs deficient skeletal progenitors.

2022-10-02 | GSE214454 | GEO

Neonatal Skeletal Progenitors

Project description:Neonatal Skeletal Progenitors

| PRJNA273968 | ENA

Neonatal Skeletal Progenitors (Affymetrix)

Project description:Neonatal Skeletal Progenitors (Affymetrix)

| PRJNA598919 | ENA

Single-cell transcriptomic analysis of embryonic vasculogenesis identifies the conversion of Etv2-deficient vascular progenitors into skeletal muscle

Project description:During vertebrate embryogenesis, vascular endothelial cells originate in the lateral plate mesoderm (LPM) next to the progenitors of skeletal muscle. It is currently not clear what prevents vascular progenitors from responding to the adjacent signals that promote muscle development. An ETS transcription factor Etv2 functions as an evolutionarily conserved master regulator of vasculogenesis. Here we performed single-cell transcriptomic analysis of hematovascular development in wild-type and etv2 mutant zebrafish embryos. Distinct transcriptional signatures of different types of hematopoietic and vascular progenitors were identified using an etv2ci32Gt gene trap line, in which Gal4 transcriptional activator has integrated into the etv2 gene locus. Unexpectedly, a cell population with the skeletal muscle signature was observed in etv2-deficient embryos. We demonstrate that multiple etv2ci32Gt; UAS:GFP cells migrate into the somites, elongate and differentiate as skeletal muscle cells instead of contributing to vasculature in etv2-deficient embryos. Wnt and FGF signaling promoted the differentiation of these putative multipotent etv2 progenitor cells into skeletal muscle cells. We conclude that etv2 actively represses muscle differentiation in vascular progenitors, thus locking these cells into vascular endothelial fate. We also identified the transcriptional signature of putative multipotent progenitors within the LPM that may give rise to vascular progenitor and skeletal muscle cells. Finally, we demonstrate that arterial progenitors co-express multiple arterial and venous markers during early stages of vasculogenesis, suggesting multi-potency of early vascular progenitors.These findings will be important in understanding the ontogeny of different mesodermal lineages and will help in designing in vitro differentiation strategies to generate vascular, muscle and other types of progenitors for therapeutic purposes.

2020-03-10 | GSE142484 | GEO

Single-cell transcriptomic analysis of embryonic vasculogenesis identifies the conversion of Etv2-deficient vascular progenitors into skeletal muscle

Project description:An ETS transcription factor Etv2 functions as an evolutionarily conserved master regulator of vasculogenesis. Here we performed single-cell transcriptomic analysis of hematovascular development in wild-type and etv2 mutant zebrafish embryos. Distinct transcriptional signatures of different types of hematopoietic and vascular progenitors were identified using an etv2ci32Gt gene trap line, in which the Gal4 transcriptional activator is integrated into the etv2 gene locus. Unexpectedly, a cell population with a skeletal muscle signature was observed in etv2-deficient embryos. We demonstrate that multiple etv2ci32Gt; UAS:GFP cells migrate into the somites, elongate and differentiate as skeletal muscle cells instead of contributing to vasculature in etv2-deficient embryos.

2020-01-16 | GSE143750 | GEO

Bulk RNAseq of HUVECs

Project description:Bulk RNAseq of HUVECs

| PRJNA862721 | ENA

Bulk RNAseq of HDLECs

Project description:Bulk RNAseq of HDLECs

| PRJNA877122 | ENA

Bulk RNAseq of control, XBP1-deficient or XBP1/IRE1-deficient cDC1s from subcutaneous B16- melanoma bearing mice

Project description:Bulk RNAseq of control, XBP1-deficient or XBP1/IRE1-deficient cDC1s from subcutaneous B16- melanoma bearing mice

| PRJNA800664 | ENA

Bulk RNAseq of HDLECs

Project description:Bulk RNAseq was performed using P4 human dermal lymphatic endothelial cells (HDLECs)