Project description:We investigate the dynamic of DNA methylation on epiblast differentiation. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation on WT murine ESCs during 3 days. We next performed bisulfite conversion and whole-genome sequecing to assess methylation levels.
Project description:We report the application of genome-wide RNA-sequencing analysis for WT or EsrrbKO mES cells after transition towards EpiLC and subsequent differentiation in early (d3) or late (d5) PGCLC. We find that formative early and formative late gene signatures were significantly reduced in EsrrbKO EpiLC compared to WT EpiLC. Furthermore, both early and late PGC gene sets were significantly downregulated in EsrrbKO PGCLC, while somatic markers were robustly increased. This indicates that the developmental program is not properly activated during transition towards PGCLC in EsrrbKO cells.
Project description:PD0325901 is involved in improving reprogramming efficiency during inducing pluripotent stem cells (iPSC) or maintain a blastocyst-like state in embryonic stem cells (ESC). However, the knowledge about this small molecule regulating miRNAs in ESC was limited. To understand the role of miRNAs during PD03-induced ESC maintenance and gain an insight how PD0325901 regulates miRNAs expression; we performed small RNA sequencing using Illumina HiSeq 2000 under the compounds treatment. The data show the miRNAs regulated by PD0325901. J1 mESCs maintained in medium containing 1000 U/mL LIF and supplemented with PD0325901 for 24 hours, J1 treated with DMSO was set as control. Then total RNA was extracted for analysis.
Project description:Pluripotency is a transient feature during early embryonic development, which enables the epiblast cells to generate all the somatic lineages of the organism. Studies using mouse embryo and embryonic stem cell (ESC) indicates that embryonic pluripotency is highly dynamic with a spectrum of pluripotent states. To better understand and characterise the different pluripotent states (Naïve, Primed and EpiLC), we have generated RNA-seq datasets.
Project description:Comparison of gene expression profiles of J1 embryonic stem cells and J1 embryoid bodies. This study should reveal genes that, when expressed, are responsible for the maintenance of the stem cell phenotype. Keywords: other
Project description:Comparison of gene expression profiles of J1 embryonic stem cells and J1 embryoid bodies. This study should reveal genes that, when expressed, are responsible for the maintenance of the stem cell phenotype. Experiment Overall Design: this experiment include 2 samples and 12 replicates
Project description:DDX6 is an RNA helicase and involved in various post-transcriptional regulatory processes. Although DDX6 is an evolutionarily well conserved central player in post-transcriptional gene regulation, its function in embryonic development remains obscure. In this study, we have identified the crucial functions of DDX6 during early mouse embryogenesis. However, due to its wide range of involvement, the more detailed dissection of DDX6-mediated functions was required to pinpoint which specific DDX6 function is important. For this purpose, we selected a key gene of each well-known DDX6 pathway, generated KO ES line, then made cDNA libraries of ESC and EpiLC Day2 samples to investigate transcriptomic differences during the very early differentiation processes of embryonic pluripotent cells.