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Unravelling the impact of aging on the human endothelial lncRNA transcriptome [HUVEC CTL_vs_PCAT14 RNA-seq]

ABSTRACT: Unravelling the impact of aging on the human endothelial lncRNA transcriptome [HUVEC CTL_vs_PCAT14 RNA-seq]

PROVIDER: PRJNA885494 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21756827.fastq.gz	Fastqsanger.gz
	SRR21756828.fastq.gz	Fastqsanger.gz
	SRR21756829.fastq.gz	Fastqsanger.gz
	SRR21756830.fastq.gz	Fastqsanger.gz
	SRR21756831.fastq.gz	Fastqsanger.gz

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Unravelling the impact of aging on the human endothelial lncRNA transcriptome

Project description:Unravelling the impact of aging on the human endothelial lncRNA transcriptome

| PRJNA885487 | ENA

Unravelling the impact of aging on the human endothelial lncRNA transcriptome [Young_vs_Aged RNA-seq]

Project description:Unravelling the impact of aging on the human endothelial lncRNA transcriptome [Young_vs_Aged RNA-seq]

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Unravelling the impact of aging on the human endothelial lncRNA transcriptome [HUVEC CTL_vs_PCAT14 RNA-seq]

Project description:Gene expression profiling analysis was performed using the RNA-Seq data obtained from endothelial cells expressing or knockdown for PCAT14

2022-10-02 | GSE214478 | GEO

Unravelling the impact of aging on the human endothelial lncRNA transcriptome.

Project description:The incidence and prevalence of cardiovascular disease is highest among the elderly. There is a need to further understand the mechanisms behind endothelial cell aging in order to achieve vascular rejuvenation and minimize the onset of age-related vascular diseases. Long non-coding RNAs (lncRNAs) have been proposed to regulate numerous processes in the human genome, yet their function in vascular aging and their therapeutic potential remain largely unknown. This is primarily because the majority of studies investigating the impact of aging on lncRNA expression heavily rely on in vitro studies based on replicative senescence. Here, using a unique collection of young and aged endothelial cells isolated from native human arteries, we sought to characterize the age-related alterations in lncRNA expression profiles. We were able to detect a total of 4463 lncRNAs expressed in the human endothelium from which ∼17% (798) were altered in advanced age. One of the most affected lncRNAs in aging was the primate-specific, Prostate Cancer Associated Transcript (PCAT) 14. In our follow up analysis, using single molecule RNA FISH, we showed that PCAT14 is relatively abundant, localized almost exclusively in the nucleus of young endothelial cells, and silenced in the aged endothelium. Functionally, our studies proposed that downregulation of PCAT14 alters endothelial cell transcription profile and cell functions including endothelial cell migration, sprouting and inflammatory responses in vitro. Taken together, our data highlight that endothelial cell aging correlates with altered expression of lncRNAs, which could impair the endothelial regenerative capacity and enhance inflammatory phenotypes.

| S-EPMC9634578 | biostudies-literature

Unravelling the impact of aging on the human endothelial lncRNA transcriptome

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-10-02 | GSE214479 | GEO

Unravelling the impact of aging on the human endothelial lncRNA transcriptome [Young_vs_Aged RNA-seq]

Project description:Bulk RNA sequncing from human native young and aged endothelial cells

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Screening for micropeptides in HUVEC without tryptic digestion, part 1

Project description:Long non-coding RNAs (lncRNA) are transcribed but not translated ribonucleic acids with various functions. We analyzed a so far unreported lncRNA n342419, which we named MANTIS. A search for micropeptides after overexpression of MANTIS in Human Umbilical Vein Endothelial Cells (HUVEC) with subsequent LC-MS/MS without trypsination showed that none of the micropeptides found had any similarity to potential MANTIS ORFs, whereas the positive control yielded micropeptides encoded from GFP plasmid.

2017-03-27 | PXD005951 | Pride

Expression profiling in Human Umbilical Vein Endothelial Cells (HUVEC)

Project description:Whole transcriptome gene expression profiling of Normal -(HUVEC) Human Umbilical Vein Endothelial Cells The HUVEC gene expression results analyzed in this study are further described in Kustermann S. et al. (2014) A real-time impedance based screening assay for drug induced vascular leakage.

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mRNA expression of HUVEC cells treated by exosomes derived from colorectal cancer cell line HCT116 transfected with lncRNA-APC1 silenced or control vector

Project description:To determine the mRNA expression profile of HUVEC cells treated by exosomes derived from colorectal cancer cell line HCT116 transfected with lncRNA-APC1 silenced or control vector, we performedd gene expression microArray analysis form Arraystar to examine the expression of mRNAs.

2019-02-07 | GSE113739 | GEO

Screening for micropeptides in HUVEC with tryptic digestion, part 2

Project description:Long non-coding RNAs (lncRNA) are transcribed but not translated ribonucleic acids with various functions. We analyzed a so far unreported lncRNAn 342419, which we named MANTIS. A search for micropeptides after overexpression of MANTIS in Human Umbilical Vein Endothelial Cells (HUVEC) with subsequent LC-MS/MS with trypsination showed that none of the micropeptides found had any similarity to potential MANTIS ORFs.

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