Proteomics

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Screening for micropeptides in HUVEC without tryptic digestion, part 1

ABSTRACT: Long non-coding RNAs (lncRNA) are transcribed but not translated ribonucleic acids with various functions. We analyzed a so far unreported lncRNA n342419, which we named MANTIS. A search for micropeptides after overexpression of MANTIS in Human Umbilical Vein Endothelial Cells (HUVEC) with subsequent LC-MS/MS without trypsination showed that none of the micropeptides found had any similarity to potential MANTIS ORFs, whereas the positive control yielded micropeptides encoded from GFP plasmid.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Ilka Wittig  

LAB HEAD: Ralf P. Brandes

PROVIDER: PXD005951 | Pride | 2017-03-27

REPOSITORIES: Pride

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161111_A1_ML747_Sample1.raw Raw
161111_A1_ML747_Sample10.raw Raw
161111_A1_ML747_Sample11.raw Raw
161111_A1_ML747_Sample12.raw Raw
161111_A1_ML747_Sample2_161115111146.raw Raw
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Publications

Sphingosine-1-Phosphate (S1P) is a potent signaling lipid. The effects of S1P are mediated by the five S1P receptors (S1PR). In the endothelium S1PR1 is the predominant receptor and thus S1PR1 abundance limits S1P signaling. Recently, lncRNAs were identified as a novel class of molecules regulating gene expression. Interestingly, the lncRNA NONHSAT004848 (LISPR1, Long intergenic noncoding RNA antisense to S1PR1), is closely positioned to the S1P1 receptors gene and in part shares its promoter re  ...[more]

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