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RNAseq expression libraries from Mycobacterium abscessus with deletion of the sRNA B11

ABSTRACT: RNAseq expression libraries from Mycobacterium abscessus with deletion of the sRNA B11

PROVIDER: PRJNA886436 | ENA |

REPOSITORIES: ENA

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RNAseq expression libraries from Mycobacterium abscessus with deletion of the sRNA B11

Project description:WT M. abscessus ATCC_19977 was used to construct a targeted deletion mutant lacking the sRNA B11 and a complemented version of the mutant with ectopic expression of B11 from its native promoter at the L5 site. The deletion mutant was also complemented with versions of B11 that lacked a single C from the first loop or had an extra C in the second loop. All strains were grown to log phase and the extracted RNA was used to perform expression profiling by RNAseq.

2022-10-05 | GSE214640 | GEO

Transcriptome Profiles of Mycobacterium marinum sRNA B11-Deleted and -Completed Strains

Project description:Understanding the regulatory roles of small RNAs (sRNAs) in Mycobacterium marinum is crucial for elucidating its pathogenesis. Here, we present transcriptome profiles of M. marinum strains with deletions and completions of sRNA B11. Through RNA sequencing analysis, we identified significant alterations in gene expression patterns between the B11-deleted and completed strains.

2024-05-01 | GSE264453 | GEO

Mass spectrometry-proteome Profiles of Mycobacterium marinum sRNA B11-Deleted and -Completed Strains

Project description:Understanding the regulatory roles of small RNAs (sRNAs) in Mycobacterium marinum is crucial for elucidating its pathogenesis. Here, we present proteome profiles of M. marinum strains with deletions and completions of sRNA B11. Through mass spectrometry analysis, we identified significant alterations in gene expression patterns between the B11-deleted and completed strains.

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Transcriptome Profiles of Mycobacterium marinum sRNA B11-Deleted and -Completed Strains

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Identification of variant distribution in ethanol-selected sRNA libraries

Project description:Identification of variant distribution in ethanol-selected sRNA libraries

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New rapid scheme for distinguishing subspecies of the Mycobacterium abscessus group and identification of Mycobacterium massiliense with inducible clarithromycin resistance

Project description:Mycobacterium abscessus [M. abscessus (sensu lato) or M. abscessus group] comprises three closely related taxa with taxonomic status under revision: M. abscessus sensu stricto, M. bolletii and M. massiliense. We describe here a simple, robust and cost effective PCR-based method for distinguishing among M. abscessus, M. massiliense and bolletii. Based on the M. abscessus ATCC 19977T genome, discriminatory regions were identified between M. abscessus and M. massiliense from array-based comparative genomic hybridization. A typing scheme using PCR primers designed for four of these locations was applied to 46 well-characterized clinical isolates comprising 29 M. abscessus, 15 M. massiliense and 2 M. bolletii previously identified by multi-target sequencing. Interestingly, 2 isolates unequivocally identified as M. massiliense were shown to have a full length erm(41) instead of the expected gene deletion and showed inducible clarithromycin resistance after 14 days. We propose using this PCR-based typing scheme combined with erm(41) PCR for a straightforward identification of M. abscessus, M. massiliense and M. bolletii and assessment of inducible clarithromycin resistance. This method can be easily implemented into a routine workflow providing subspecies level identification within 24 hours of isolation of M. abscessus group. Two-color CGH with 4 independent Mycobacterium clinical isolates and the M massiliense type strain (CCUG 48898) labeled with Cy3 were cohybridized with the M abscessus type strain (ATCC 19977) labeled with Cy5 on a tiling array designed against the M abscessus type strain

2013-07-17 | E-GEOD-43629 | biostudies-arrayexpress

New rapid scheme for distinguishing subspecies of the Mycobacterium abscessus group and identification of Mycobacterium massiliense with inducible clarithromycin resistance

2013-07-17 | GSE43629 | GEO

Barcode sequencing of Saccharomyces cerevisiae deletion libraries, SYBARIS project

Project description:Barcode sequencing of Saccharomyces cerevisiae deletion libraries, SYBARIS project

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Human pluripotent stem cell-derived macrophages host Mycobacterium abscessus infection

Project description:Human macrophages are a natural host of many mycobacterium species, including Mycobacterium abscessus (M. abscessus), an emerging pathogen affecting patients with lung diseases and immunocompromised individuals. There are few available treatments and the search for effective antibiotics against M. abscessus has been hindered by the lack of a tractable in vitro intracellular model of infection. Here, we established a reliable model for M. abscessus infection using human pluripotent stem cell-derived macrophages (hPSC-macrophages). hPSC differentiation permitted a reproducible generation of functional human macrophages that were highly susceptible to M. abscessus infection. Electron microscopy demonstrated that M. abscessus was present in the vacuoles of hPSC-macrophages. RNA-sequencing analysis revealed a time dependent host cell response to M. abscessus, with differing gene and protein expression patterns observed at 3-hours, 24-hours and 48-hours post-infection. Culture of engineered tdTOMATO-expressing hPSC-macrophages with GFP-expressing M. abscessus enabled rapid and image-based high-throughput analysis of intracellular infection and quantitative assessment of antibiotic resistance and efficacy. Our study describes the first hPSC-based model for M. abscessus infection, which represents a novel platform for studying M. abscessus-host interaction and an accessible tool for drug discovery.

2022-08-10 | GSE207456 | GEO

Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoebae and macrophages

Project description:We report the transcriptome of M. abscessus in amoebae and macrophages. M. abscessus intra-amoebal and intra-macrophagic transcriptomes demonstrate of the potential of M. abscessus to adapt to an intracellular lifestyle, though amoebae largely contribute to the enhancement of M. abscessus survival macrophages.

2019-09-20 | GSE124972 | GEO

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