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ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion [CRISPR-Cas9 gRNA screen]

ABSTRACT: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion [CRISPR-Cas9 gRNA screen]

PROVIDER: PRJNA886447 | ENA |

REPOSITORIES: ENA

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ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion [CRISPR-Cas9 gRNA screen]

Project description:The ATR kinase, which coordinates cellular responses to DNA replication stress, is also essential for the proliferation of normal unstressed cells. Although its role in the replication stress response is well defined, the mechanisms by which ATR supports normal cell proliferation remain elusive. Here, we show that Atr is dispensable for the viability of G0-arrested naïve B cells. However, upon cytokine-induced proliferation, Atr-deficient B cells initiate DNA replication efficiently in early S phase, but by mid-S phase they display dNTP depletion, fork stalling, and replication failure. Nonetheless, productive DNA replication can be restored in Atr-deficient cells by pathways that suppress origin firing, such as downregulation of CDC7 and CDK1 kinase activities. Together, these findings indicate that ATR supports the proliferation of normal unstressed cells by tempering the pace of origin firing during the early S phase to avoid exhaustion of dNTPs and other replication factors.

2022-12-31 | GSE214643 | GEO

ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion [HTGTS]

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ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion [RNA-seq]

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ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion.

Project description:ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion.

| PRJNA874443 | ENA

ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion [HTGTS]

Project description:ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion [HTGTS]

| PRJNA874445 | ENA

ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion [RNA-seq]

Project description:ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion [RNA-seq]

| PRJNA874444 | ENA

ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion.

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-12-31 | GSE212196 | GEO

RNASeq of Arg2 gRNA or scrambled gRNA CRISPR'd Tregs

Project description:RNASeq of Arg2 gRNA or scrambled gRNA CRISPR'd Tregs

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2008-04-01 | E-MEXP-1329 | biostudies-arrayexpress

Cas9 gRNA engineering for selectable genome editing, activation and repression

Project description:RNA-Seq after Cas9-gRNA transfection with different length gRNAs we performed PolyA Selection and RNA-Seq on cells transfected with dCas9-VPR and a gRNA of each length (20nt, 16nt, or 14nt) targeting ACTC1, MIAT, or HBG1/2

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