Project description:Transcriptional profiling of splenic lymphocytes derived from vaccinated mice, and ex-vivo exposed to M.tb.-infected macrophages in culture. Splenocytes from mice innoculated with Mycobacterium bovis BCG Pasteur (PAS), or M. bovis BCG Copenhagen (SSI), or heat killed BCG SSI (HK), or uninfected control, were ex vivo co-cultured with mouse bone marrow macrophages previously infected with M. tb.
Project description:Novel SCD inhibitor SSI-4 demonstrated good toxicity profile in experimental animals and induced cell toxicity across multiple acute myeloid leukemia models. Therefore, we treated sensitive AML cell line MV-4-11 with SSI-4 (1 µM) for 24h with or without the addition of sodium oleate (1 µM) that rescues toxicity phenotype.
Project description:Antibiotic lavage reduces bacterial contamination and decreases SSI infection rate. SSI leads to an immunocompromised situation, leaving unattended the neoplasm. It has been described that SSI may result in a worse oncologic outcome.