Ontology highlight
ABSTRACT:
PROVIDER: PRJNA887074 | ENA |
REPOSITORIES: ENA
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Project description:Hakea laurina, a woody plant in Proteaceae family, naturally occurs in severely phosphorus (P)-impoverished environments in southwest Australia. It develops distinctive “cluster roots” that exhibit a high capacity for carboxylate exudation and acid phosphatase activity, contributing to P acquisition. However, the molecular mechanisms underlying these physiological functions remain poorly understood. We explored the cluster root transcriptome using de novo RNA-Seq, which detected homologous transcripts of an aluminum (Al)-activated malate transporter (ALMT), designated HalALMT1, and two multidrug and toxic compound extrusion (MATE) proteins specifically expressed in mature cluster roots. Deferentially-expressed genes highlighted multiple carboxylate-related processes at maturity. Electrophysiological assays and overexpressed transgenic experiments confirmed HalALMT1’s ability to release malate and that its activity is enhanced by exposure to Al3+. Notably, HalALMT1 was specifically expressed in the mature cortex cells of cluster rootlets, which do not develop a suberized exodermis. Furthermore, acid phosphatase activity was pronounced throughout the rootlets, whereas it was only present in the epidermis and stele of non-cluster roots, revealing its extensive production in the rootlets. These results suggest that malate release and acid phosphatase production in the dominant cortex cells in cluster rootlets increase the amount exuded. This study sheds light on an outstanding P-acquisition strategy of Proteaceae.
2024-12-31 | GSE279190 | GEO
Project description:Phylogenomics of Artocarpus and select Moraceae from HybSeq (hybridization-based target enrichment) data. Raw sequence reads
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