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Gene expression profiles in the colonic epithelial cells from mice deficient in the function of St6galnac6, B3galt5 and B3gnt7

ABSTRACT: Gene expression profiles in the colonic epithelial cells from mice deficient in the function of St6galnac6, B3galt5 and B3gnt7

PROVIDER: PRJNA887413 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21815942.fastq.gz	Fastqsanger.gz
	SRR21815943.fastq.gz	Fastqsanger.gz
	SRR21815944.fastq.gz	Fastqsanger.gz
	SRR21815945.fastq.gz	Fastqsanger.gz
	SRR21815946.fastq.gz	Fastqsanger.gz

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Gene expression profiles in the colonic epithelial cells from mice deficient in the function of St6galnac6, B3galt5 and B3gnt7

Project description:To investigate the effect of deficiency of glycosyltransferases on the intestinal epithelial cells, we analyzed the gene expression profiles in colonic epithelial cells of mice deficient in the function of St6galnac6, B3galt5 and B3gnt7.

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Gene expression profiles in the ileal and colonic epithelial cells from Interleukin (IL)-23-deficient mice and IL-22-deficient mice

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Project description:We analyzed the RNA expression pattern in B3GALT5-Knockout H9 cells (B3GALT5-KO) relative to wild type H9 cells (WT) by RNA-seq. Collectively, our results reveal that B3GALT5-Knockout cells cultured in 2iLAF condition represent naïve-specific characteristics as well as WT cells convert to naïve state in 5iLAF condition. These findings support that loss of B3GALT5 which result in depletion of primed-specific GSLs not only do not have influence on maintain pluripotency and ability to specific lineage differentiation but also could promotes naive pluripotency generation in hESCs.

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Gene expression profiles in the ileal and colonic epithelial cells from Interleukin (IL)-23-deficient mice and IL-22-deficient mice

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Profiling the DNA methylation pattern in B3GALT5 knockout hESCs cultured in basal and 2iLAF media

Project description:We analyzed the DNA methylation pattern in B3GALT5-Knockout H9 cells (B3GALT5-KO) relative to wild type H9 cells (WT) by whole-genome bisulfite sequencing. Collectively, our results reveal that B3GALT5-Knockout cells cultured in 2iLAF condition represent naïve-specific characteristics as well as WT cells convert to naïve state in 5iLAF condition. These findings support that loss of B3GALT5 which result in depletion of primed-specific GSLs not only do not have influence on maintain pluripotency and ability to specific lineage differentiation but also could promotes naive pluripotency generation in hESCs.

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