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Post-transcriptional regulation of Ribosome Biogenesis during differentiation

ABSTRACT: Post-transcriptional regulation of Ribosome Biogenesis during differentiation

PROVIDER: PRJNA887436 | ENA |

REPOSITORIES: ENA

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Post-transcriptional regulation of Ribosome Biogenesis during differentiation [dRNA-seq]

Project description:Ribosome biogenesis is a critical component of cell differentiation. Ribosome synthesis has been previously reported to be highly regulated at the transcriptional level, but less is known about its post-transcriptional regulation. Poly(A) tail length regulation is a hallmark of post-transcriptional regulation associated with transcript stability. Here we monitor poly(A) tail length changes at a transcriptome level during P19 differentiation. We found that poly(A) tail shortening occurs during cell differentiation only for transcript encoding for ribosomal proteins. These findings suggest a strong post-transcriptional regulation of ribosome biogenesis during differentiation.

2023-08-04 | GSE214874 | GEO

Post-transcriptional regulation of Ribosome Biogenesis

Project description:Post-transcriptional regulation of Ribosome Biogenesis

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Post-transcriptional regulation of Ribosome Biogenesis during differentiation [SLAM-seq]

Project description:The regulation of ribosome biogenesis is a critical component of cell differentiation. Although ribosome biogenesis is well known to be regulated at the transcriptional level, its post-transcriptional regulation is less well understood. A key component of RNA post-transcriptional regulation is transcript decay rate which, together with the rate of synthesis, defines the abundance of the transcripts. Here we measured the stability of the whole transcriptome of P19 cells before differentiation (D0) and six days after the onset of differentiation (D6). We found that while global decay rates dropped during differentiation, the stability of transcripts encoding for ribosomal proteins remains significantly high throughout the differentiation process. These results indicate a strong and specific post-transcriptional regulation of ribosome biogenesis transcripts that is sustained during cell differentiation.

2023-08-04 | GSE214860 | GEO

Post-transcriptional regulation of Ribosome Biogenesis during differentiation [SLAM-seq]

Project description:Post-transcriptional regulation of Ribosome Biogenesis during differentiation [SLAM-seq]

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Post-transcriptional regulation of Ribosome Biogenesis during differentiation [dRNA-seq]

Project description:Post-transcriptional regulation of Ribosome Biogenesis during differentiation [dRNA-seq]

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Post-transcriptional regulation of Ribosome Biogenesis in Larp1-deficient cells

Project description:Post-transcriptional regulation of Ribosome Biogenesis in Larp1-deficient cells

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Post-transcriptional regulation of Ribosome Biogenesis upon treatment with MHY1485

Project description:Post-transcriptional regulation of Ribosome Biogenesis upon treatment with MHY1485

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Post-transcriptional regulation of Ribosome Biogenesis upon treatment with MHY1485

Project description:Ribosome biogenesis is a critical aspect of cell differentiation. Ribosome synthesis has been previously reported to be regulated at the transcriptional and post-transcriptional levels. Poly(A) tail-length processing is a hallmark of post-transcriptional regulation associated with different steps of transcript metabolism. Here we monitor the contribution of mTOR pathway activation upon treatment with the agonist MHY1485 in shaping the poly(A) tail profile of undifferentiated P19 cells. We found that transcripts with a 5' terminal oligopyrimidine (TOP) motif, including those encoding for ribosomal proteins, specifically accumulate with poly(A) tails ~60 nucleotides long upon mTOR activation.

2023-08-04 | GSE231978 | GEO

Post-transcriptional regulation of Ribosome Biogenesis in Larp1-deficient cells

Project description:Ribosome biogenesis is a critical aspect of cell differentiation. Ribosome synthesis has been previously reported to be regulated at the transcriptional and post-transcriptional levels. Poly(A) tail-length processing is a hallmark of post-transcriptional regulation associated with different steps of transcript metabolism. Here we monitor the contribution of the RNA-binding protein Larp1 in shaping the poly(A) tail profile of undifferentiated P19 cells. We found that Larp1 prevents the widespread shortening of poly(A) tails below 30 nucleotides and confers additional protection to transcripts containing a 5' terminal oligopyrimidine (TOP) motif, such as those encoding for ribosomal proteins.

2023-08-04 | GSE231976 | GEO

p53 activation during ribosome biogenesis regulates normal erythroid differentiation. [expression]

Project description:The role of ribosome biogenesis in erythroid development is supported by the recognition of erythroid defects in ribosomopathies in both Diamond-Blackfan anemia and 5q- syndrome. Whether ribosome biogenesis exerts a regulatory function on normal erythroid development is still unknown. In the present study, a detailed characterization of ribosome biogenesis dynamics during human and murine erythropoiesis shows that ribosome biogenesis is abruptly interrupted by the drop of rDNA transcription and the collapse of ribosomal protein neo-synthesis. Its premature arrest by RNA polI inhibitor, CX-5461 targets the proliferation of immature erythroblasts. We also show that p53 is activated spontaneously or in response to CX-5461 concomitantly to ribosome biogenesis arrest, and drives a transcriptional program in which genes involved in cell cycle arrest, negative regulation of apoptosis and DNA damage response were upregulated. RNA polI transcriptional stress results in nucleolar disruption and activation of ATR-CHK1-p53 pathway. Our results imply that the timing of ribosome biogenesis extinction and p53 activation are crucial for erythroid development. In ribosomopathies in which ribosome availability is altered by unbalanced production of ribosomal proteins, the threshold of ribosome biogenesis down-regulation could be prematurely reached and together with pathological p53 activation prevents a normal expansion of erythroid progenitors.

2020-09-14 | GSE137951 | GEO

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