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Post-transcriptional regulation of Ribosome Biogenesis during differentiation

ABSTRACT: Post-transcriptional regulation of Ribosome Biogenesis during differentiation

PROVIDER: PRJNA887436 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21815400.fastq.gz	Fastqsanger.gz
	SRR21815401.fastq.gz	Fastqsanger.gz
	SRR21815402.fastq.gz	Fastqsanger.gz
	SRR21815403.fastq.gz	Fastqsanger.gz
	SRR21815404.fastq.gz	Fastqsanger.gz

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Post-transcriptional regulation of Ribosome Biogenesis during differentiation [dRNA-seq]

Project description:Ribosome biogenesis is a critical component of cell differentiation. Ribosome synthesis has been previously reported to be highly regulated at the transcriptional level, but less is known about its post-transcriptional regulation. Poly(A) tail length regulation is a hallmark of post-transcriptional regulation associated with transcript stability. Here we monitor poly(A) tail length changes at a transcriptome level during P19 differentiation. We found that poly(A) tail shortening occurs during cell differentiation only for transcript encoding for ribosomal proteins. These findings suggest a strong post-transcriptional regulation of ribosome biogenesis during differentiation.

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Post-transcriptional regulation of Ribosome Biogenesis during differentiation [SLAM-seq]

Project description:The regulation of ribosome biogenesis is a critical component of cell differentiation. Although ribosome biogenesis is well known to be regulated at the transcriptional level, its post-transcriptional regulation is less well understood. A key component of RNA post-transcriptional regulation is transcript decay rate which, together with the rate of synthesis, defines the abundance of the transcripts. Here we measured the stability of the whole transcriptome of P19 cells before differentiation (D0) and six days after the onset of differentiation (D6). We found that while global decay rates dropped during differentiation, the stability of transcripts encoding for ribosomal proteins remains significantly high throughout the differentiation process. These results indicate a strong and specific post-transcriptional regulation of ribosome biogenesis transcripts that is sustained during cell differentiation.

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Post-transcriptional regulation of Ribosome Biogenesis upon treatment with MHY1485

Project description:Ribosome biogenesis is a critical aspect of cell differentiation. Ribosome synthesis has been previously reported to be regulated at the transcriptional and post-transcriptional levels. Poly(A) tail-length processing is a hallmark of post-transcriptional regulation associated with different steps of transcript metabolism. Here we monitor the contribution of mTOR pathway activation upon treatment with the agonist MHY1485 in shaping the poly(A) tail profile of undifferentiated P19 cells. We found that transcripts with a 5' terminal oligopyrimidine (TOP) motif, including those encoding for ribosomal proteins, specifically accumulate with poly(A) tails ~60 nucleotides long upon mTOR activation.

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Project description:Ribosome biogenesis is a critical aspect of cell differentiation. Ribosome synthesis has been previously reported to be regulated at the transcriptional and post-transcriptional levels. Poly(A) tail-length processing is a hallmark of post-transcriptional regulation associated with different steps of transcript metabolism. Here we monitor the contribution of the RNA-binding protein Larp1 in shaping the poly(A) tail profile of undifferentiated P19 cells. We found that Larp1 prevents the widespread shortening of poly(A) tails below 30 nucleotides and confers additional protection to transcripts containing a 5' terminal oligopyrimidine (TOP) motif, such as those encoding for ribosomal proteins.

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