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ScRNA-seq/CITE-seq analysis of aortic CD45+ cells from mice expressing Jak2V617F mutation in myeloid cells

ABSTRACT: scRNA-seq/CITE-seq analysis of aortic CD45+ cells from mice expressing Jak2V617F mutation in myeloid cells

PROVIDER: PRJNA887459 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21819685_1.fastq.gz	Fastqsanger.gz
	SRR21819685_2.fastq.gz	Fastqsanger.gz
	SRR21819686_1.fastq.gz	Fastqsanger.gz
	SRR21819686_2.fastq.gz	Fastqsanger.gz
	SRR21819687.fastq.gz	Fastqsanger.gz

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scRNA-seq/CITE-seq analysis of aortic CD45+ cells from mice expressing Jak2V617F mutation in myeloid cells

Project description:JAK2V617F mutation is associated with an increased risk for athero-thrombotic cardiovascular disease, but its role in aortic disease development and complications remains unknown. In a cohort of patients with myeloproliferative neoplasm, JAK2V617F mutation was identified as an independent risk factor for dilation of both the ascending and descending thoracic aorta. Using single-cell RNA-seq, complementary genetically-modified mouse models, as well as pharmacological approaches, we found that JAK2V617F mutation was associated with a pathogenic pro-inflammatory phenotype of perivascular tissue-resident macrophages, which promoted deleterious aortic wall remodeling at early stages, and dissecting aneurysm through the recruitment of circulating monocytes at later stages. Finally, genetic manipulation of tissue-resident macrophages, or treatment with a Jak2 inhibitor, ruxolitinib, mitigated aortic wall inflammation and reduced aortic dilation and rupture. Overall, JAK2V617F mutation drives vascular resident macrophages toward a pathogenic phenotype and promotes dissecting aortic aneurysm.

2022-10-26 | GSE214880 | GEO

scRNA-seq of mouse CD45+ aortic cells with CITE-seq and cell Hashing after continuous or alternate diet

Project description:scRNA-seq of mouse CD45+ aortic cells with CITE-seq and cell Hashing after continuous or alternate diet

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scRNA-seq of mouse cardiac CD45+ cells with CITE-seq and cell Hashing

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scRNA-seq of mouse cardiac CD45+ cells with CITE-seq and cell Hashing II

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scRNA-seq of mouse CD45+ aortic cells with CITE-seq and cell Hashing after continuous or alternate diet

Project description:scRNA-seq of mouse CD45+ aortic cells with CITE-seq and cell Hashing after continuous or alternate diet

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JAK2V617F-dependent clonal hematopoiesis drives vascular resident macrophages toward a pathogenic phenotype and promotes dissecting aortic aneurysm

Project description:In order to understand the mechanisms responsible for lethal JAK2V617F-dependent aortic disease, we performed RNA sequencing in the aorta of 7-week old Jak2V617F HC-EC and control Jak2WT mice

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scRNA-seq of mouse cardiac CD45+ cells with CITE-seq and cell Hashing

Project description:scRNA-seq of mouse cardiac CD45+ cells with CITE-seq and cell Hashing

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Single cell RNA sequencing of aortic CD45+ cells and foam cells from atherosclerotic aorta

Project description:In atherosclerosis, several immune cells are involved in plaque formation. Foam cell formation is a major cellular process in atherosclerotic lesion. It is important to understand which cells participate in foam cell formation. To characterize the immune cells and foam cells in atherosclerotic aorta, we performed single cell RNA sequencing of aortic CD45+ leukocytes from Ldlr-/- mice and foamy cells from ApoE-/- mice. The single cell RNA-seq analyses revealed the heterogeneity of aortic macrophages and foam cells in atherosclerotic aorta.

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Illumina microarray analysis of three subpopulations of murine aortic adventitial cells freshly sorted by MACS, based on expression of Sca-1 and CD45

Project description:Comparison of gene expression between aortic adventitial Sca-1+CD45+ progenitor cells and Sca-1-CD45+ or Sca-1+CD45- cells. The hypothesis tested was that Sca-1+CD45+ cells would differentially express genes relating to angiogenesis and vasculogenesis when compared to Sca-1-CD45+ leukocytes.

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