Transcriptomics

Dataset Information

0

ScRNA-seq/CITE-seq analysis of aortic CD45+ cells from mice expressing Jak2V617F mutation in myeloid cells


ABSTRACT: JAK2V617F mutation is associated with an increased risk for athero-thrombotic cardiovascular disease, but its role in aortic disease development and complications remains unknown. In a cohort of patients with myeloproliferative neoplasm, JAK2V617F mutation was identified as an independent risk factor for dilation of both the ascending and descending thoracic aorta. Using single-cell RNA-seq, complementary genetically-modified mouse models, as well as pharmacological approaches, we found that JAK2V617F mutation was associated with a pathogenic pro-inflammatory phenotype of perivascular tissue-resident macrophages, which promoted deleterious aortic wall remodeling at early stages, and dissecting aneurysm through the recruitment of circulating monocytes at later stages. Finally, genetic manipulation of tissue-resident macrophages, or treatment with a Jak2 inhibitor, ruxolitinib, mitigated aortic wall inflammation and reduced aortic dilation and rupture. Overall, JAK2V617F mutation drives vascular resident macrophages toward a pathogenic phenotype and promotes dissecting aortic aneurysm.

ORGANISM(S): Mus musculus

PROVIDER: GSE214880 | GEO | 2022/10/26

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2020-03-01 | GSE140947 | GEO
2020-03-01 | GSE141031 | GEO
2017-03-31 | MSV000080837 | MassIVE
2016-12-23 | PXD003702 | Pride
2024-02-14 | PXD037853 | Pride
2021-08-19 | PXD027125 | Pride
| PRJNA146571 | ENA
2016-12-01 | GSE67182 | GEO
2023-08-30 | GSE237229 | GEO
2024-09-19 | GSE239620 | GEO