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ABSTRACT: Effect of heat stress and bacterial inoculation on Symbiodiniaceae microbiome

PROVIDER: PRJNA887545 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21820400_1.fastq.gz	Fastqsanger.gz
	SRR21820400_2.fastq.gz	Fastqsanger.gz
	SRR21820500_1.fastq.gz	Fastqsanger.gz
	SRR21820500_2.fastq.gz	Fastqsanger.gz
	SRR21820401_1.fastq.gz	Fastqsanger.gz

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Differential transcriptomic responses to heat in wild-type and heat-evolved Symbiodiniaceae in corals

Project description:Heat-evolved Symbiodiniaceae can improve the physiological performances of their coral host under heat stress, but their gene expression responses to heat remained unknown. We explore here the transcriptomic basis of differential thermal stress responses between in hospite wild-type and heat-evolved Cladocopium proliferum strains and their coral host Platygyra daedealea.

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The role acclimatization, adaptation, and symbiosis in the acquisition of heat tolerance in reef-building corals

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Project description:The gut microbiome is significantly altered in inflammatory bowel diseases, but the basis of these changes is not well understood. We have combined metagenomic and metatranscriptomic profiling of the gut microbiome to assess changes to both bacterial community structure and transcriptional activity in a mouse model of colitis. Gene families involved in microbial resistance to oxidative stress, including Dps/ferritin, Fe-dependent peroxidase and glutathione S-transferase, were transcriptionally up-regulated in colitis, implicating a role for increased oxygen tension in gut microbiota modulation. Transcriptional profiling of the host gut tissue and host RNA in the gut lumen revealed a marked increase in the transcription of genes with an activated macrophage and granulocyte signature, suggesting the involvement of these cell types in influencing microbial gene expression. Down-regulation of host glycosylation genes further supports a role for inflammation-driven changes to the gut niche that may impact the microbiome. We propose that members of the bacterial community react to inflammation-associated increased oxygen tension by inducing genes involved in oxidative stress resistance. Furthermore, correlated transcriptional responses between host glycosylation and bacterial glycan utilisation support a role for altered usage of host-derived carbohydrates in colitis. Complementary transcription profiling data from the mouse hosts have also been deposited at ArrayExpress under accession number E-MTAB-3590 ( http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-3590/ ).

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