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Spatial transcriptome unveils the heterogeneity between subpopulations of Bacillus subtilis surface-associated communities

ABSTRACT: Spatial transcriptome unveils the heterogeneity between subpopulations of Bacillus subtilis surface-associated communities

PROVIDER: PRJNA887802 | ENA |

REPOSITORIES: ENA

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Spatial transcriptome unveils the heterogeneity between subpopulations of Bacillus subtilis surface-associated communities

Project description:Bacillus subtilis has been extensively used as a model for molecular studies on biofilm formation. These studies encompassed the development of complex macro-colonies on agar, the formation of pellicles at the air-liquid interface, and lately the formation of submerged architectural biofilms at the solid-liquid interface. Beside similarities, these multicellular communities also display considerable heterogeneity at the structural, chemical and biological levels. Here we use RNA-seq to analyze nine different spatio-physiological conditions, including the three biofilm populations (colony, pellicle, and submerged).

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Computational identification of surface markers for isolating distinct subpopulations from heterogeneous cancer cell populations [scRNA-seq]

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