Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bulk RNA sequecing reveals the heterogeneity of fibroblasts in the diabetic heart

ABSTRACT: Bulk RNA sequecing reveals the heterogeneity of fibroblasts in the diabetic heart

PROVIDER: PRJNA891630 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21943973_1.fastq.gz	Fastqsanger.gz
	SRR21943973_2.fastq.gz	Fastqsanger.gz
	SRR21943974_1.fastq.gz	Fastqsanger.gz
	SRR21943974_2.fastq.gz	Fastqsanger.gz
	SRR21943975_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Bulk RNA sequecing reveals the heterogeneity of fibroblasts in the diabetic heart

2023-01-31 | GSE215979 | GEO

Single cell analysis reveals the heterogeneity of fibroblasts in the diabetic heart

Project description:Single cell analysis reveals the heterogeneity of fibroblasts in the diabetic heart

| PRJNA880548 | ENA

Single cell transcriptome sequencing of diabetic mouse heart

Project description:Cardiovascular disease is the leading cause of mortality in individuals with diabetes. However, the specific mechanisms and cell heterogeneity underlying cardiac injury in diabetes are still unclear, making it difficult to effectively manage diabetic cardiovascular damage. Our study used leptin receptor knockout (db/db) mice as diabetic models and sequenced their hearts at different pathological stages. Our findings provide important insights for preventing and treating diabetic heart damage.

2024-10-31 | GSE243789 | GEO

Single cell analysis reveals the heterogeneity of fibroblasts in the diabetic heart

Project description:Diabetes mellitus is one of the most chronic diseases, of which diabetic cardiomyopathy is the major cause of morbidity and involves in multiple processes such as inflammation, oxidative stress, fibrosis, extracellular collagen deposition, apoptosis, mitochondria dysfunction. However, the exact mechanisms of fibroblasts concerning type Ⅰ diabetes remain unclear. To further understand the functional roles of fibroblasts of STZ-induced diabetic mice, we lead the single cell RNA sequence. Cells were comprised of endothelial, fibroblast, cardiomyocyte, smooth muscle cells, macrophage and other type cells. Single cell sequence illustrates novel fibroblast sub-clusters and highlight the role of Lox. Real-time quantitative PCR, western blotting, immunofluorescence was used to verify the sequence data. We validate the dysfunctions of diabetic cardiomyopathy by echocardiogram. Our study supports novel insights into the pathogenesis of diabetic cardiomyopathy.

2023-01-31 | GSE213337 | GEO

Global gene expression analysis of developing heart in embryos of diabetic mice

Project description:Maternal diabetes causes cardiac malformations in fetuses. In this study, we have analyzed the differential gene expression profiling in the developing heart of embryos from diabetic and control mice by using the oligonucleotide microarray. Expression patterns of genes and proteins that are differentially expressed in the developing heart were further examined by the real-time reverse transcriptase-polymerase chain reaction and immunohistochemistry. Embryos of diabetic pregnancies displayed cardiac malformations. Microarray analysis revealed the genes that were altered in expression level in the developing heart of embryos from diabetic mice when compared to controls. It is concluded that altered expression of a variety of genes involved in heart development is associated with cardiac malformations in offsprings of diabetic mother. We used microarrays to identify the genes specific to the developing heart of embryos from control and diabetic mice RNA was isolated from heart tissue of control and diabetes exposed E13.5 and E15.5 mouse embryos (three samples each). The RNA was hybridised onto Affymetrix Mouse Genome 430 2.0 Array.

2011-09-13 | E-GEOD-32078 | biostudies-arrayexpress

Transcriptome analysis of diabetic and non diabetic patients affected by post-ischemic heart failure

Project description:Increased morbidity and mortality associated with post-ischemic heart failure (HF) in diabetic patients underscore the need for a better understanding of the underlying molecular events. Indeed, effective HF therapy in diabetic patients requires a complex strategy encompassing the development of improved diagnostic and prognostic markers and innovative pharmacological approaches. Whole mRNAs expression was measured in the heart of patients with heart failure (HF) with or without concomitant Type 2 diabetes mellitus (T2DM) and compared it to control non-failing hearts. We identified distinct genes modulated in HF patients compared to controls, as well as to T2DM HF patients compared to not diabetic HF patients. Our study included left ventricle (LV) cardiac biopsies taken from the vital, non-infarcted zone (remote zone) derived from patients affected by dilated hypokinetic post-ischemic cardiomyopathy, undergoing surgical ventricular restoration procedure. Inclusion criteria for diabetic were: GLICEMIA: >=126 mg/dl, previous T2DM diagnosis or anti-diabetic therapy, while for non diabetic: GLICEMIA: <100 mg/dl and HbA1c: n.v. 4.8-6.0%. Moreover, HF patients were matched for End Systolic Volume (ESV), Ejection fraction (LVEF), Age, Sex, Ethnic distribution, Smoke habits, Hypertension, Glomerular filtration rate (GFR), Body Mass Index (BMI). Genes expression was assessed by Affymetrix GeneChips Human Gene 1.0 ST array, using total RNA extracted from 7 T2DM HF patients, 12 non-T2DM HF patients and 5 controls.

2012-03-21 | E-GEOD-26887 | biostudies-arrayexpress

Transcriptome analysis of diabetic and non diabetic patients affected by post-ischemic heart failure

2012-03-21 | GSE26887 | GEO

Molecular characterization of diabetic heart of akita (array)

Project description:To understand the molecular profile of diabetic heart in animal model of type 1 diabeties Akita, we performed RNA sequencing of heart tissue (male) at the 12 week to understand systemic changes.

2015-09-30 | GSE66576 | GEO

Molecular characterization of diabetic heart of akita

Project description:Molecular characterization of diabetic heart of akita

| PRJNA277353 | ENA

Transcriptome Alteration in the Diabetic Heart by Rosiglitazone: Implications for Cardiovascular Mortality

Project description:The type 2 diabetes medication, rosiglitazone, has come under scrutiny for possibly increasing the risk of cardiac disease and death. To investigate the effects of rosiglitazone on the diabetic heart, we performed cardiac transcriptional profiling of a murine model of type 2 diabetes, the C57BL/KLS-leprdb/leprdb (db/db) mouse. We compared cardiac gene expression profiles from three groups: untreated db/db mice (db-c), db/db mice after rosiglitazone treatment (db-t), and non-diabetic db/+ mice. Mice were divided into three groups: Non-diabetic controls (db/+), untreated diabetic controls (db-c), and rosiglitazone-treated diabetic mice (db-t). Whole-heart RNA from five mice from each of the three groups after four months with or without treatment was used for microarray analysis.Universal Reference RNAs for mouse (Stratagene, La Jolla, CA) were purchased as microarray reference controls.

2012-03-28 | E-GEOD-36875 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data