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Comparative analysis of genome-wide transcriptional responses to continuous heat stress in Pleurotus tuoliensis

ABSTRACT: Comparative analysis of genome-wide transcriptional responses to continuous heat stress in Pleurotus tuoliensis

PROVIDER: PRJNA892610 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21986371_1.fastq.gz	Fastqsanger.gz
	SRR21986371_2.fastq.gz	Fastqsanger.gz
	SRR21986372_1.fastq.gz	Fastqsanger.gz
	SRR21986372_2.fastq.gz	Fastqsanger.gz
	SRR21986373_1.fastq.gz	Fastqsanger.gz

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Comparative analysis of genome-wide transcriptional responses to continuous heat stress in Pleurotus tuoliensis

Project description:The whole-genome gene expression profiling was performed by RNA-Seq after 96 h HS treatment of P. tuoliensis mycelia. Divergent mycelial growth rates were observed under the HS with different intensities, which facilitated comparative analyses combining both physiological and transcriptomic data. The goal aimed to acquire a deeper insight into the genome-wide gene expression patterns during the response to HS in mushrooms.

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A comparative transcriptome analysis reveals physiological maturation properties of mycelia in Pleurotus tuoliensis

Project description:Pleurotus tuoliensis is a precious edible fungus with extremely high nutritive and medicinal value. The cultivation period of P. tuoliensis is longer than those of other Pleurotus species, which is mainly due to a longer mycelium physiological maturation period (30-60 days). Currently, the molecular processes underlying physiological maturation of the mycelium remain unclear. We performed a comparative transcriptomic analysis of immature and mature mycelia using RNA-seq. De novo transcriptome assembly resulted in identification of 17,030 unigenes. 451 differentially expressed genes, including those encoding nucleoside diphosphate kinase (NDPK), glycoside hydrolase family proteins, exopolygalacturonase, and versatile peroxidases, were identified. GO and KEGG analyses revealed that nucleotide synthesis and energy metabolism are highly active during the physiological maturation of mycelia, and genes related to these pathways were significantly up-regulated in mature mycelia. NDPK is predicted to be essential for mycelia maturation. Our findings contribute to a comprehensive understanding of mycelia maturation in a commercially important fungal species. Future efforts will focus on the function of NDPK and the mechanism by which it regulates mycelia maturation.

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Comparative analysis of genome-wide transcriptional responses to continuous heat stress in Pleurotus tuoliensis.

Project description:Temperature plays an impactful role in mushroom cultivation. To obtain insights of transcriptomic response in macrofungi against heat stress, we performed RNA-seq analysis using Pleurotus tuoliensis mycelium cells that were treated under 32 °C and 36 °C for consecutive 96 h. By comparing the growth rate data, we found mycelium cells could maintain normal growth rate almost the same as control under 32 °C, yet halted the growths under 36 °C. In total, 2724 differential expressed genes were identified from the three pair-wise comparisons, which were classified to four clusters based on their expression patterns. We also performed gene set enrichment analysis using both GO and KEGG databases, and revealed 48, 113 and 105 enriched GO terms, and 1, 5, and 6 enriched KEGG pathways for three pair-wise comparisons accordingly. In addition, we identified 9 overlapping GO terms and 1 overlapping KEGG pathway shared by the three comparisons. Differentially expressed genes (DEGs) involved in cell communication, amino acid metabolic process, intracellular signal transduction and small molecule biosynthesis were identified in two heat stress treatments despite of the stress intensity. However, the expression of two heat shock protein genes (HSP10 and HSP60) were induced by increasing temperature. Our findings also suggested the DEGs associated with cell cycle regulation had various expression patterns under two heat stress conditions possibly due to different functions. Furthermore, 11 DEGs related to ergosterol biosynthesis were identified with similar expression trends, indicating the ergosterol levels and cell membrane composition may have a tight connection to the acquisition of thermotolerance, which warrant further investigations for deeper understanding of molecular mechanisms in fungal stress responses.

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Project description:Pleurotus tuoliensis strain:CCMJ1044 Transcriptome or Gene expression