Project description:Members of the bacterial phylum Spirochaetes are primarily studied for their commensal and pathogenic roles in animal hosts. However, Spirochaetes are also frequently detected in anoxic hydrocarbon-contaminated environments but their ecological role in such ecosystems has so far remained unclear. Here we provide a functional trait to these frequently detected organisms with an example of a sulfate-reducing, naphthalene-degrading enrichment culture consisting of a sulfate-reducing deltaproteobacterium Desulfobacterium naphthalenivorans and a novel spirochete Rectinema cohabitans. Using a combination of genomic, proteomic, and physiological studies we show that R. cohabitans grows by fermentation of organic compounds derived from biomass from dead cells (necromass). It recycles the derived electrons in the form of H2 to the sulfate-reducing D. naphthalenivorans, thereby supporting naphthalene degradation and forming a simple microbial loop. We provide metagenomic evidence that equivalent associations between Spirochaetes and hydrocarbon-degrading microorganisms are of general importance in hydrocarbon- and organohalide-contaminated ecosystems. We propose that environmental Spirochaetes form a critical component of a microbial loop central to nutrient cycling in subsurface environments. This emphasizes the importance of necromass and H2-cycling in highly toxic contaminated subsurface habitats such as hydrocarbon-polluted aquifers.

2018-05-17 | PXD005624 | Pride

Surface prokaryotic community in China Sea

Project description:Surface prokaryotic community in China Sea Raw sequence reads

| PRJNA1005344 | ENA

Prokaryotic community structure of bioaugmented anaerobic digester

Project description:Prokaryotic community structure of bioaugmented anaerobic digester Metagenome

| PRJNA904670 | ENA

Polluted soil

Project description:Polluted soil

| PRJNA1063465 | ENA

Dynamics of prokaryotic community in pit muds

Project description:Cellar age related dynamics of prokaryotic community in pit muds