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Achatina immaculata

ABSTRACT: Achatina immaculata Raw sequence reads

PROVIDER: PRJNA894328 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR22141245_1.fastq.gz	Fastqsanger.gz
	SRR22141245_2.fastq.gz	Fastqsanger.gz
	SRR22141246_1.fastq.gz	Fastqsanger.gz
	SRR22141246_2.fastq.gz	Fastqsanger.gz
	SRR22141247_1.fastq.gz	Fastqsanger.gz

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Exome sequencing in classic Hairy cell leukaemia reveals widespread variation in acquired somatic mutations between individual tumours apart from the signature BRAF V(600)E lesion

Project description:Whole exome sequencing of 5 HCLc tumor-germline pairs. Genomic DNA from HCLc tumor cells and T-cells for germline was used. Whole exome enrichment was performed with either Agilent SureSelect (50Mb, samples S3G/T, S5G/T, S9G/T) or Roche Nimblegen (44.1Mb, samples S4G/T and S6G/T). The resulting exome libraries were sequenced on the Illumina HiSeq platform with paired-end 100bp reads to an average depth of 120-134x. Bam files were generated using NovoalignMPI (v3.0) to align the raw fastq files to the reference genome sequence (hg19) and picard tools (v1.34) to flag duplicate reads (optical or pcr), unmapped reads, reads mapping to more than one location, and reads failing vendor QC.

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High-throughput sequencing reveals a simple model of nucleosome energetics

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