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Antigen-expanded CD8+ gd T cells with NK-like phenotype and function respond in persistent infection [scRNA-seq]

ABSTRACT: Antigen-expanded CD8+ gd T cells with NK-like phenotype and function respond in persistent infection [scRNA-seq]

PROVIDER: PRJNA894703 | ENA |

REPOSITORIES: ENA

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Antigen-expanded CD8+ gd T cells with NK-like phenotype and function respond in persistent infection

Project description:Antigen-expanded CD8+ gd T cells with NK-like phenotype and function respond in persistent infection

| PRJNA894700 | ENA

Antigen-expanded CD8+ gd T cells with NK-like phenotype and function respond in persistent infection [Bulk RNA-seq]

Project description:Antigen-expanded CD8+ gd T cells with NK-like phenotype and function respond in persistent infection [Bulk RNA-seq]

| PRJNA894702 | ENA

Antigen-expanded CD8+ gd T cells with NK-like phenotype and function respond in persistent infection [scRNA-seq]

Project description:Gamma Delta (gd) T cells play unique protective roles in different settings of acute and chronic infections. However, what they respond to and how they function in the acute versus chronic phases of the same infection remains unclear. It is well documented that cytolytic gd T cells that express Vg9Vd2 TCRs and respond to phosphoantigens (pAgs) expand in acute Mycobacterium tuberculosis (Mtb) infection in humans. However, most infections with Mtb manifest as a chronic, clinically asymptomatic state. While past work indicates that gd T cells contribute to Mtb infection control, their response during this phase of Mtb infection is not well understood. Here, we analyze peripheral blood gd T cells from a South African adolescent cohort and show that a unique CD8+ gd T cell subset with features of “memory inflation” expand in chronic Mtb infection. Using targeted scRNA-seq coupled with surface protein expression and multiparametric single cell pseudotime trajectory analysis, we identify the differentiation paths that these CD8+ gd T cells follow to develop into effectors in this infection state.

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Antigen-expanded CD8+ gd T cells with NK-like phenotype and function respond in persistent infection [Bulk RNA-seq]

2022-10-31 | GSE216652 | GEO

Antigen-expanded CD8+ gd T cells with NK-like phenotype and function respond in persistent infection

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-10-31 | GSE216658 | GEO

A comparison of the transcriptional profiles of resting NK cells and ex vivo expanded NK cells

Project description:NK cells are cytotoxic lymphocytes that play an important role in the innate immune response. The immune response of NK cells was shown under activated conditions. In order to identify such patterns, we performed RNA sequencing to confirm the gene expression profiles in NK cells expanded ex vivo compared to that of resting NK cells.

2023-05-17 | GSE222740 | GEO

IFNAR1 Signaling in NK Cells Promotes Persistent Virus Infection

Project description:Inhibition of IFN-I signaling promotes the control of persistent virus infection, but the underlying mechanisms remain poorly understood. Here we report that genetic ablation of IFNAR1 specifically in NK cells led to elevated numbers of T follicular helper cells, germinal center B cells, and plasma cells, resulting in hastened virus clearance comparable to IFNAR1 blockade by an IFNAR1 neutralizing antibody. Antigen-specific B cells and antiviral antibodies were essential for the accelerated control of LCMV Cl13 infection following IFNAR1 blockade. IFNAR1 signaling in NK cells promoted NK cell maturation, function, and killing of antigenspecific CD4 and CD8 T cells. Therefore, Inhibition of IFN I signaling in NK cells enhances CD4 and CD8 T cell responses, elevates humoral immune response, and thereby facilitates the control of persistent virus infection.

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RNAseq of freshly isolated and ex vivo expanded peripheral blood NK cells

Project description:NK cells were isolated from the perpheral blood of healthy donors (n=6) and expanded ex vivo in the presence of feeder cells and IL-2. RNA from fresh and expanded cells was isolated, sequenced, and gene expression of the cell populations were compared.

2021-03-10 | GSE165498 | GEO

PBS vs. LPS stimulation of gd T cells

Project description:gd T cells recognize unprocessed or non-peptide antigens, respond rapidly to infection, and localize to mucosal surfaces. We have hypothesized that the innate functions of gd T cells may be more similar to those of cells of the myeloid lineage than to other T cells. To begin to test this assumption, we have analyzed the direct response of cultured human and peripheral blood bovine gd T cells to pathogen associated molecular patterns (PAMPs) in the absence of APCs using microarray, real time RT-PCR, proteome array, and chemotaxis assays. Our results indicate that purified gd T cells respond directly to PAMPs by increasing expression of chemokine and activation related genes. The response was distinct from that to known gd T cell antigens and different from the response of myeloid cells to PAMPs. In addition, we have analyzed the expression of a variety of PAMP receptors in gd T cells. Freshly purified bovine gd T cells responded more robustly to PAMPs than did cultured human cells and expressed measurable mRNA encoding a variety of PAMP receptors. Our results suggest that rapid response to PAMPs through the expression of PAMP receptors may be another innate role of gd T cells. Keywords: parallel sample

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