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TBX3 is dynamically expressed in pancreatic organogenesis and fine-tunes regeneration

ABSTRACT: TBX3 is dynamically expressed in pancreatic organogenesis and fine-tunes regeneration

PROVIDER: PRJNA896121 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22105361.fastq.gz	Fastqsanger.gz
	SRR22105362.fastq.gz	Fastqsanger.gz
	SRR22105363.fastq.gz	Fastqsanger.gz
	SRR22105364.fastq.gz	Fastqsanger.gz
	SRR22105365.fastq.gz	Fastqsanger.gz

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TBX3 is dynamically expressed in pancreatic organogenesis and fine-tunes regeneration

Project description:We investigated transcriptional profiles of Ptf1a-Cre driven homozygous Tbx3-KO and wildtype pancreata 72h and 168h after onset of acute pancreatitis.

2023-02-24 | GSE216889 | GEO

TBX3 is dynamically expressed in pancreatic organogenesis and fine-tunes regeneration.

Project description:BackgroundThe reactivation of genetic programs from early development is a common mechanism for injury-induced organ regeneration. T-box 3 (TBX3) is a member of the T-box family of transcription factors previously shown to regulate pluripotency and subsequent lineage commitment in a number of tissues, including limb and lung. TBX3 is also involved in lung and heart organogenesis. Here, we provide a comprehensive and thorough characterization of TBX3 and its role during pancreatic organogenesis and regeneration.ResultsWe interrogated the level and cell specificity of TBX3 in the developing and adult pancreas at mRNA and protein levels at multiple developmental stages in mouse and human pancreas. We employed conditional mutagenesis to determine its role in murine pancreatic development and in regeneration after the induction of acute pancreatitis. We found that Tbx3 is dynamically expressed in the pancreatic mesenchyme and epithelium. While Tbx3 is expressed in the developing pancreas, its absence is likely compensated by other factors after ablation from either the mesenchymal or epithelial compartments. In an adult model of acute pancreatitis, we found that a lack of Tbx3 resulted in increased proliferation and fibrosis as well as an enhanced inflammatory gene programs, indicating that Tbx3 has a role in tissue homeostasis and regeneration.ConclusionsTBX3 demonstrates dynamic expression patterns in the pancreas. Although TBX3 is dispensable for proper pancreatic development, its absence leads to altered organ regeneration after induction of acute pancreatitis.

| S-EPMC10029195 | biostudies-literature

Tbx3 controls Dppa3 levels to regulate exit from pluripotency towards mesodermal lineage

Project description:Tbx3, a member of the T-box family, plays important roles in development, stem cells, nuclear reprogramming and cancer. Loss of Tbx3 induces differentiation in mouse embryonic stem cells (mESCs). However, we show that mESCs exist in an alternate stable pluripotent state in the absence of Tbx3. In-depth transcriptome analysis of this mESC state reveals Dppa3 as a direct downstream target of Tbx3. Also Tbx3 facilitates the cell fate transition from pluripotent cells to mesoderm progenitors by directly repressing Wnt pathway members required for differentiation. Wnt signaling regulates differentiation of mESCs into mesoderm progenitors and helps maintain a naïve pluripotent state. We show that Tbx3, a downstream target of Wnt signaling, fine-tunes these divergent roles of Wnt signaling in mESCs. In conclusion, we identify a signaling-TF axis that controls the exit of mESCs from a self-renewing pluripotent state towards mesoderm differentiation. ChIPSeq and RNASeq (population and single cell) was performed on the indicated cell lines. Replicates are indicated as needed. The mm9 genome assembly was used. For single cell mRNA-Seq preparation, SSEA1+DAPI- mESCs were sorted and collected.

2015-07-21 | E-GEOD-60066 | biostudies-arrayexpress

Tbx3 controls Dppa3 levels to regulate exit from pluripotency towards mesodermal lineage

2015-07-21 | GSE60066 | GEO

Mnx1 fine tunes neuronal gene expression in motor neurons

Project description:Mnx1 fine tunes neuronal gene expression in motor neurons

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Mecp2 fine-tunes quiescence exit by targeting nuclear receptors

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A dynamic role of Tbx3 in the pluripotency circuitry

Project description:Pluripotency represents a cell state comprising a fine-tuned pattern of transcription factor activity required for embryonic stem cell (ESC) self-renewal. TBX3 is the earliest expressed member of the T-box transcription factor family and is involved in maintenance and induction of pluripotency. Hence, TBX3 is believed to be a key member of the pluripotency circuitry, with loss of TBX3 coinciding with loss of pluripotency. We report a dynamic expression of TBX3 in vitro and in vivo using genetic reporter tools tracking TBX3 expression in mouse ESCs (mESCs). Low TBX3 levels are associated with reduced pluripotency, resembling the more mature epiblast. Notably, TBX3-low cells maintain the intrinsic capability to switch to a TBX3-high state and vice versa. Additionally, we show TBX3 to be dispensable for induction and maintenance of naive pluripotency as well as for germ cell development. These data highlight novel facets of TBX3 action in mESCs. 12 samples in total

2015-12-09 | E-GEOD-73862 | biostudies-arrayexpress

A PP2A-Integrator complex fine-tunes transcription by opposing CDK9

Project description:A PP2A-Integrator complex fine-tunes transcription by opposing CDK9

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A dynamic role of Tbx3 in the pluripotency circuitry

2015-12-09 | GSE73862 | GEO

Mecp2 fine-tunes quiescence exit by targeting nuclear receptors [ChIP-Seq]

Project description:Mecp2 fine-tunes quiescence exit by targeting nuclear receptors [ChIP-Seq]

| PRJNA946720 | ENA