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RGS4 impacts carbohydrate and siderophore metabolism in Trichoderma reesei

ABSTRACT: RGS4 impacts carbohydrate and siderophore metabolism in Trichoderma reesei

PROVIDER: PRJNA896230 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22114140_1.fastq.gz	Fastqsanger.gz
	SRR22114140_2.fastq.gz	Fastqsanger.gz
	SRR22114141_1.fastq.gz	Fastqsanger.gz
	SRR22114141_2.fastq.gz	Fastqsanger.gz
	SRR22114142_1.fastq.gz	Fastqsanger.gz

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RGS4 impacts carbohydrate and siderophore metabolism in Trichoderma reesei

Project description:We investigated the function of the SNX/H-type regulator of G-protein signaling (RGS) protein RGS4 and found alterations in enzyme regulation, stress response, siderophore production and metabolism of several carbon sources in light and darkness

2022-12-12 | GSE216955 | GEO

RGS4 impacts carbohydrate and siderophore metabolism in Trichoderma reesei.

Project description:BackgroundAdaptation to complex, rapidly changing environments is crucial for evolutionary success of fungi. The heterotrimeric G-protein pathway belongs to the most important signaling cascades applied for this task. In Trichoderma reesei, enzyme production, growth and secondary metabolism are among the physiological traits influenced by the G-protein pathway in a light dependent manner.ResultsHere, we investigated the function of the SNX/H-type regulator of G-protein signaling (RGS) protein RGS4 of T. reesei. We show that RGS4 is involved in regulation of cellulase production, growth, asexual development and oxidative stress response in darkness as well as in osmotic stress response in the presence of sodium chloride, particularly in light. Transcriptome analysis revealed regulation of several ribosomal genes, six genes mutated in RutC30 as well as several genes encoding transcription factors and transporters. Importantly, RGS4 positively regulates the siderophore cluster responsible for fusarinine C biosynthesis in light. The respective deletion mutant shows altered growth on nutrient sources related to siderophore production such as ornithine or proline in a BIOLOG phenotype microarray assay. Additionally, growth on storage carbohydrates as well as several intermediates of the D-galactose and D-arabinose catabolic pathway is decreased, predominantly in light.ConclusionsWe conclude that RGS4 mainly operates in light and targets plant cell wall degradation, siderophore production and storage compound metabolism in T. reesei.

| S-EPMC10316542 | biostudies-literature

Expression analysis of Trichoderma reesei QM9414 and delta-phlp1, delta-gnb1 and delta-gng1 in light and darkness.

Project description:Investigation of whole genome gene expression level changes in response to different light conditions of the T. reesei QM9414 parental strain and the deletion strains delta-phlp1, delta-gnb1 and delta gng1, cultivated on 1 % microcrystalline cellulose. The mutants analyzed in this study are further described in Tisch et al. 2011: Carbohydrate degradation is significantly regulated by light and the phosducin like protein PhLP1 in Trichoderma reesei (Hypocrea jecorina).

2011-03-02 | GSE27581 | GEO

Expression analysis of Trichoderma reesei QM9414 and delta-phlp1, delta-gnb1 and delta-gng1 in light and darkness.

2011-03-02 | E-GEOD-27581 | biostudies-arrayexpress

Trichoderma reesei QM6a CGH self hybridization

Project description:We perform a self hybridisation comprative genomic hybridization (CGH) in order to validate the probe tiling design we done on Trichoderma reesei. This hybridization was done using QM6a wild type strain.

2010-03-16 | GSE17752 | GEO

Trichoderma reesei transcript levels during mycoparasitism

Project description:To investigate the potential role in mycoparasitism, microarrays were used to examine T. reesei transcript levels when confronted with a potential prey (the plant pathogen Rhizoctonia solani) before contact, during first physical contact and during overgrowth of the host.

2011-07-01 | GSE23410 | GEO

Trichoderma reesei QM6a CGH self hybridization

2010-06-23 | E-GEOD-17752 | biostudies-arrayexpress

Identification of microRNA-like RNAs in the filamentous fungus Trichoderma reesei by Solexa sequencing

Project description:MicroRNAs (miRNAs) are small non-coding RNAs capable of negatively regulating gene expression. Trichoderma reesei is an industrial filamentous fungus that can secrete abundant hydrolases for cellulosic biofuels. Recently, microRNA-like RNAs (milRNAs) were discovered in several filamentous fungi rather than T. reesei. The purpose of this study was to explore the presence of milRNA in T. reesei, to characterize the differential expression of T. reesei milRNA under cellulose induction, and to reveal the target genes of milRNA involved in cellulase production. Two small RNA libraries of cellulose induction (IN) or non-induction (CON) were generated and sequenced using Solexa sequencing technology. A total of 664,463 and 529,545 unique sequences, representing 1,271 and 1,021 unique small RNAs, were obtained from the IN and CON samples, respectively. Thirteen milRNAs were finally identified in T. reesei using the hairpin structure analysis. The milRNAs profiles obtained in deep sequencing were validated by RT-qPCR assay. The miRanda program predicts a number of potential targets for T. reesei milRNAs, including several hydrolases and carbon catabolite repressor Cre1.The presence and differential expression of T. reesei milRNAs, along with their predicted targets indicate that milRNAs might play a regulatory role in cellulase induction. This work lays foundation for further functional study of fungal milRNAs and their industrial application.

2014-12-31 | GSE46679 | GEO

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