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ABSTRACT: Gene transcription of hESC derived trophoblast 72h after B7-H4/VTCN1 Kockdown

PROVIDER: PRJNA897811 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22164500_1.fastq.gz	Fastqsanger.gz
	SRR22164500_2.fastq.gz	Fastqsanger.gz
	SRR22164501_1.fastq.gz	Fastqsanger.gz
	SRR22164501_2.fastq.gz	Fastqsanger.gz
	SRR22164502_1.fastq.gz	Fastqsanger.gz

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Project description:Gene transcription of hESC derived trophoblast 24h after B7-H4/VTCN1 Kockdown

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Project description:Gene transcription of hESC derived trophoblast 48h after B7-H4/VTCN1 Kockdown

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The immune checkpoint molecule, B7-H4/VTCN1, guides differentiation and suppresses proinflammatory responses and MHC class I expression in an embryonic stem cell-derived model of primitive human trophoblast III

Project description:VTCN1/B7-H4 is expressed in human embryonic stem cells (hESC) to an in vitro model of primitive trophoblast (TB) and first trimester but not term human placenta and that primitive trophoblast may be uniquely susceptible to certain pathogens.

2023-03-13 | GSE227096 | GEO

2023-03-13 | GSE227095 | GEO

2023-03-13 | GSE227094 | GEO

B7-H4 regulates β cell mass and insulin secretion by modulating cholesterol metabolism

Project description:Chronic islet inflammation is a hallmark of type 2 diabetes (T2D) and involves in the dysfunction of β cells. However, how β cells participate in this process remains unclear. Here, we report that the immune checkpoint molecule B7-H4(B7S1, B7x, VTCN1) expressed in β cells is critical to maintain β cell mass and insulin secretion. Lesion of B7-H4 in β cells results in glucose intolerance due to less β cell mass and deficient insulin secretion with upregulated cytokines and activated signal transducer and activator of transcription 5 (Stat5) signaling, while overexpression of B7-H4 in β cells ameliorates glucose intolerance in high-fat diet (HFD)-treated mice. Mechanistically, B7-H4 deficiency actives the Stat5 signaling, which inhibits the expression of Apolipoprotein F (ApoF), leading to reduced cholesterol efflux and accumulated cholesterol in β cells, thereby impairing the insulin processing and secretion. Inhibiting Stat5 activity or overexpression of ApoF in β cells can rescue the glucose intolerance and insulin secretion deficiency in β-cell-specific B7-H4 knockout (B7-H4 cKO) mice. Our study demonstrates that β cell expressed immune checkpoint molecule B7-H4 is essential for islet immune homeostasis and β cell function maintenance, and for the first time unravels the mechanism by which B7-H4 regulates insulin secretion through regulating cholesterol metabolism via Stat5 signaling, which may shed new light on the development of novel strategies for T2D treatment.

2024-12-19 | GSE268688 | GEO

Microinjection of human ESC-derived trophoblast cells(BAP cells) to mouse blastocysts

Project description:Purpose: 1. Characterize hESCs and hESC-derived trophoblast cells Results: 1. hESCs have high level of most pluripotency markers; 2. hESC-derived trophoblast cells specifically express trophoblast markers.

2024-11-22 | GSE224029 | GEO

Analysis of Wild Type and B7-H4-/- leukemia initial cells Transcriptomes

Project description:The goal of this study are to find the abonormally expressed genes of B7-H4-/- leukemia inital cells in MLL-AF9 induced AML mice by RNA-seq analysis.Methods: Lin- festival liver cells were isolated from WT and B7-H4-null mice and infected with MLL-AF9 retroviruses, then transplanted to the lethally irradiated recipient mice. When AML developed, we isolated the YFP+/Mac-1+/c-Kit+ bone marrow cells by FACS and and then made the RNA-sequence using Illumina. Results: We identified 27180 transcripts in wild type and B7-H4-/- leukemia initial cells. 572 transcripts showed differential expression between the WT and B7-H4−/− LICs, with a fold change ≥1.5 and p value <0.01. 271 transcripts were downregulated and 241 transcripts were upregulated.

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Genome wide mapping of chromatin binding sites of GATA2, GATA3, TFAP2A, TFAP2C transcription factors in trophoblast progenitors derived from human embryonic stem cells

Project description:Human embryonic stem cells (hESC) can be differentiated into progenitors resembling trophoblast upon exposure to BMP4. Among the earliest transcription factors that are activated after the BMP4 stimulation are GATA2, GATA3, TFAP2A and TFAP2C. Using trophoblast progenitors at day 3 of BMP4-induced differentiation, here we profile the chromatin binding landscape of these 4 early transcription factors to analyse their putative targets and cross-connectivity in regualtion of trophoblast commitment.

2017-10-18 | GSE105081 | GEO

Expression data from APA+ and APA- progenitors and SSEA5+ human pluripotent stem cells

Project description:Human embryonic stem cells (hESC) can be differentiated into progenitors resembling trophoblast upon exposure to BMP4. Putative trophpblast progenitors express APA cell surface marker Using FACS sorting and microarray we analysed the gene expression of APA-positive trophoblast progenitors, APA-negative progenitors and SSEA5-positive undifferentiated hESC and identified genes that contribute to the trophoblast fate

2017-10-12 | GSE104818 | GEO

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