Project description:We performed transcriptome analysis in A4368-treated cells to investigate the effect A4368 on genome-wide expression in Hep3B cells.
Project description:Overexpression of SOX4 in various kinds of cancers specimen was associated with poor prognosis of patients; however, the role of SOX4 in angiogenesis or tumor microenvironment modulation remains unclear. Therefore the endogenous SOX4 was knockout and the differential gene expression between Hep3B and Hep3B SOX4-/- cells were examined via genechip. We found that the differentially expressed genes, EzH2, a SOX4-associated partner, and CXCL12, were repressed in Hep3B SOX4-/- cells compared with parental Hep3B; these results were further assessed via qRT-PCR in Hep3B SOX4-/- versus Hep3B cells.
Project description:To investigate the associated pathway of CSPF6, we established a hep3B cell line in which the target gene was broken down by siRNA.
Project description:WIPI2 regulates the growth of human liver cancer cells. We used microarray to detail the global program of gene expression underlying the cell growth of human liver cancer cell line Hep3b cells.
Project description:We were interested in identifying novel splice junctions in Hep3B cells, especially in genes involved in cholesterol metabolism like HMGCR, so we sequenced polyA-tailed RNA from Hep3B cells. 1 polyA-selected RNA sample was sequenced from Hep3B cells