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Gene expression profile at single cell level of phagocytic myeloid cells in autochthonous lung cancer model

ABSTRACT: Gene expression profile at single cell level of phagocytic myeloid cells in autochthonous lung cancer model

PROVIDER: PRJNA898629 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22199977.fastq.gz	Fastqsanger.gz
	SRR22199978.fastq.gz	Fastqsanger.gz
	SRR22199979.fastq.gz	Fastqsanger.gz
	SRR22199980.fastq.gz	Fastqsanger.gz
	SRR22199981.fastq.gz	Fastqsanger.gz

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Gene expression profile at single cell level of phagocytic myeloid cells in autochthonous lung cancer model

Project description:Phagocytosis is a critical process undertaken by myeloid cells in the tumor microenvironment. We used single-cell RNA-seq (scRNAseq) to investigate molecular and cellular consequences of neoplastic cell engulfment using a tdTomato genetically engineered mouse model.

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Project description:To detect total RNA in microglia derived from AD mice (5xFAD) brain we single cell sorted Cd11b+ and Cd45+ cells divided by Methoxy-X04 staining (Ab aggregates) positivity We performed differentially expression analysis of RNA-seq of wild-type microglia (Me-X04 neg), non-phagocytic AD microglia (Me-X04 neg), phagocytic AD microglia (Me-X04 positive)

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