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ABSTRACT: Mutation accumulation (MA) study of Mycobacterium smegmatis mc2 155 wild type and nucS-deficient strains

PROVIDER: PRJNA898800 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22207065_1.fastq.gz	Fastqsanger.gz
	SRR22207065_2.fastq.gz	Fastqsanger.gz
	SRR22207066_1.fastq.gz	Fastqsanger.gz
	SRR22207066_2.fastq.gz	Fastqsanger.gz
	SRR22207067_1.fastq.gz	Fastqsanger.gz

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Project description:Investigation of whole genome gene expression level changes in a Mycobacterium smegmatis mc2 155 delta-MSMEG_0166 mutant, compared to the wild-type strain. MSMEG_0166 is a transcriptional regulator in the gntR family. Mutations in MSMEG_0166 result in hypersensitivity to the bactericidal ubiquitin peptide Ub2, as well as oxidative stress caused by either organic or inorganic agents A three chip study using total RNA recovered from three separate wild-type cultures of Mycobacterium smegmatis mc2 155 and three separate cultures of a MSMEG_0166 mutant strain, Mycobacterium smegmatis mc2 155 delta-MSMEG_0166, in which MSMEG_0166 is interupted by the insertion of a hygromycin resistance cassette. Each chip measures the expression level of 6,588 genes from Myobacterium smegmatis with five 60-mer probes per transcript and two replicates of each probe for a total of 65,880 experimental probes.

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