Project description:A major driver of neural circuit complexity is cellular heterogeneity. We sought to profile all of the neural cell types in the Drosophila embryo at stage 17, a period of rapid synapse and circuit formation in the central nervous system. To do so, we performed whole embryo dissociations of Drosophila embryos followed by single cell RNA sequencing. We then computationally segregated neural cell types based on known gene expression for all neurons (elav) and glia (repo). We hope that this dataset will serve as a community resource for other researchers interested in the molecular determinants of circuit complexity.
Project description:In this study we use a combination of proteomics Label-Free quantification methods to monitor protein expression changes over a time course of more than 20 hours of embryo development in Drosophila melanogaster.
Project description:A spectral library was built for Drosophila melanogaster. The spectral library allows reproducible quantification for thousands of peptides per SWATH-MS analysis.
Proteins from Drosophila melanogaster embryo, adult flies were digested with trypsin using in-gel digestion and the peptides were fractionated by high-pH reverse phase chromatography. HRM peptides were spiked into the peptides mixture and each fraction was injected on a Sciex TripleTOF 6600 mass spectrometer fitted with microflow set-up.
The resulting .wiff files were analysed using MaxQuant and Spectronaut.
