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Transcriptomic analysis of leaves and leaf cells from the medicinal plant Catharanthus roseus

ABSTRACT: Transcriptomic analysis of leaves and leaf cells from the medicinal plant Catharanthus roseus

PROVIDER: PRJNA901021 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22267200_1.fastq.gz	Fastqsanger.gz
	SRR22267201_1.fastq.gz	Fastqsanger.gz
	SRR22267202_1.fastq.gz	Fastqsanger.gz
	SRR22267203_1.fastq.gz	Fastqsanger.gz
	SRR22267204_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of leaves and leaf cells from the medicinal plant Catharanthus roseus

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Project description:This dataset belongs to a set of three RNA-Seq experiments that were carried out to study the regulation of monoterpenoid indole alkaloid production in the medicinal plant Catharanthus roseus. For this dataset, C. roseus flower petals were infiltrated with Agrobacterium tumefaciens C58C1 or infiltration buffer as control. For each sample, flower petals from four to five flowers, each from a different individual plant were infiltrated.

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Project description:Catharanthus roseus produces a variety of indole alkaloids with significant biological activities. The indole alkaloids including catharanthine, vindolinine, ajmalicine and the precursor strictosidine were dramatically induced in the leaves following binary stress. To profile the modification of indole alkaloids in C. roseus seedlings under the binary stress of ultraviolet-B irradiation and dark incubation, gel-free proteomic analysis was carried out to uncover the underlying molecular mechanism.

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Project description:In this study, we aim to present a global transcriptome analysis of medicinal plant, Catharanthus roseus. We generated about 343 million high-quality reads from three tissues (leaf, root and flower) using Illumina platform. We performed an optimized de novo assembly of the reads and estimated transcript abundance in different tissue samples. The transcriptome dynamics was studied by differential gene expression analyses among tissue samples.

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