Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The effects of ifosfamide and citalopram on developing zebrafish

ABSTRACT: The effects of ifosfamide and citalopram on developing zebrafish

PROVIDER: PRJNA901165 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22270138_1.fastq.gz	Fastqsanger.gz
	SRR22270138_2.fastq.gz	Fastqsanger.gz
	SRR22270139_1.fastq.gz	Fastqsanger.gz
	SRR22270139_2.fastq.gz	Fastqsanger.gz
	SRR22270140_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 40

Similar Datasets

The effects of broflanilide on developing zebrafish

Project description:The effects of broflanilide on developing zebrafish

| PRJNA991461 | ENA

The effects of atorvastatin (AVS) and afidopyropen on developing zebrafish

Project description:The effects of atorvastatin (AVS) and afidopyropen on developing zebrafish

| PRJNA931595 | ENA

The effects of ifosfamide and citalopram on developing zebrafish

Project description:For RNA-seq experiments, 20 embryos were placed into 5 mL final volume of sterile ERM. Each treatment group were exposed within a 10-minute window. Exposure groups included ERM, 1 µg/L, and 100 µg/L ifosfamide or 10 and 1000 µg/L citalopram (N=5 beakers per treatment). On 7 days post fertilization (dpf), fish were transferred to a labeled and pre-weighed 1.7 mL tube using a p1000 with the tip cut off, then water was completely removed using a p200. The tube was then immediately flash frozen in liquid nitrogen prior to RNA-seq analysis.

2023-01-02 | GSE217875 | GEO

The proteomics effects of HSP60 chaperone deficiency in zebrafish larvae

Project description:To understand the effects of Hsp60 deficiency in developing vertebrates, we generated CRISPR/Cas9-mediated hspd1 knockout zebrafish lines by targeting exon 2 to induce a frameshift mutation. We selected an allele with a 56 base pair deletion inducing a frameshift mutation leading to loss of protein functions. We examined the proteome changes in zebrafish larvae at 5 days post fertilization (DPF). Wildtype control and hspd1-/- larvae at 5dpf, were analyzed by TMT and nanoLC-MS/MS based proteomcis. For this purpose, we studied five pools from each genotype, and each pool consisted of five larvae.

2024-09-27 | PXD048920 | Pride

Functional heterogeneity within the developing zebrafish epicardium

Project description:Functional heterogeneity within the developing zebrafish epicardium

| PRJNA498309 | ENA

The effects of HSP60 chaperone deficiency on zebrafish larvae

2024-09-27 | GSE254091 | GEO

Effects of LXR activation in zebrafish larvae

Project description:The Liver X Receptors (LXRs) play important roles in multiple metabolic pathways, including fatty acid, cholesterol, carbohydrate and energy metabolism. To expand the knowledge of the functions of LXR signaling during embryonic development, we performed a whole-genome microarray analysis of Lxr target genes in zebrafish larvae treated with either one of the synthetic LXR ligands T0901317 or GW3965. Assessment of the biological processes enriched by differentially expressed genes revealed a prime role for Lxr in regulating lipid metabolic processes, similarly to the function of LXR in mammals. In addition, exposure to the Lxr ligands induced changes in expression of genes in the neural retina and lens of the zebrafish eye, including the photoreceptor guanylate cyclase activators and lens gamma crystallins, suggesting a potential novel role for Lxr in modulating the transcription of genes associated with visual function in zebrafish. The regulation of expression of metabolic genes was phenotypically reflected in an increased absorption of yolk in the zebrafish larvae, and changes in the expression of genes involved in visual perception were associated with morphological alterations in the retina and lens of the developing zebrafish eye. The regulation of expression of both lipid metabolic and eye specific genes was sustained in 1 month old fish. The transcriptional networks demonstrated several conserved effects of LXR activation between zebrafish and mammals, and also identified potential novel functions of Lxr, supporting zebrafish as a promising model for investigating the role of Lxr during development.

2016-03-07 | GSE59031 | GEO

A single cell transcriptomic atlas identifies synapse-associated microglia in the developing zebrafish (single cells)

Project description:This study profiles developing and adult zebrafish macrophages and defines regional and functional microglial subtypes.

2021-08-23 | GSE164772 | GEO

A single cell transcriptomic atlas identifies synapse-associated microglia in the developing zebrafish (bulk cells)

Project description:This study profiles developing and adult zebrafish macrophages and defines regional and functional microglial subtypes.

2021-08-23 | GSE164771 | GEO

Epigenetic effects of antidepressants to neuroprotective genes in neuronal cultured cells

Project description:Antidepressants are widely used for treatment of depression. Imipramine, a typical antidepressant, is recently known to have an effect to restore the expression of brain-derived neurotrophic factor gene, which is decreased in depressed patients, by increasing histone acetylation. However, it is largely unknown whether other antidepressants have similar epigenetic effects to change gene expression. To address this question, we examined the effect of five antidepressants (imipramine, citalopram, duloxetine, amitriptyline, mirtazapine) on expression of genes associated with mental and neurodegenetative disorders. We found that 48-hour treatment with imipramine, citalopram, amitriptyline, mirtazapine, but not duloxetine, caused a large number of differentially expressed genes in primary neocortical neurons in mice. Focused on genes relating to neural protection and neuroplasticity, we discovered that amitriptyline not only enhanced the expression of atf3 and cox2 by increasing histone H3 Lys4 trimethylation (H3K4me3), but also protected neurons against oxygen-glucose deprivation and staurosporine-induced apoptosis. Our study will shed a new insight in understanding of the molecular and epigenetic mechanism of antidepressants. Mouse primary neuronal cultures were used to evaluate 48 hour effects of antidepressants. Briefly, cultured neurons at day in vitro 3 (DIV3) were treated with 5ÂµM imipramine, 10 ÂµM citalopram, 0.1 ÂµM duloxetine, 5 ÂµM amitriptylline, 50 ÂµM mirtazepine. These cultures were harvested after 48 hours (DIV5).

2015-01-30 | E-GEOD-63005 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data