Project description:We propose a custom pipeline for combining data from experiments on multispecies studies tested on data from human and bovine peripheral blood mononuclear cellssamples, stimulated with Mycobacterium tuberculosis.
Project description:Comprehensively compare the transcriptional difference in PPD stimulated PBMCs from individuals with different tuberculosis infectious status: tuberculosis patients, latent infectious individuals and healthy controls using the microarray analysis.
Project description:Comprehensively compare the transcriptional difference in PPD stimulated PBMCs from individuals with different tuberculosis infectious status: tuberculosis patients, latent infectious individuals and healthy controls using the microarray analysis. Two-condition experiment, PBMCs vs. PPD-PBMCs. 12 individuals: 4 TB patients, 4 latent infectious individuals and 4 healthy controls.
Project description:To further understand the host immune factors involved in the progression from latent tuberculosis infection (LTBI) to active tuberculosis (TB) and identify the potential signatures for discriminating TB from LTBI, the genome-wide transcriptional profile of the Mycobacterium.Tuberculosis (M.TB)–specific antigens stimulated peripheral blood mononuclear cells (PBMCs) from active TB, LTBI and health controls (HCs) were performed. A total of 209- and 234- differentially expressed genes (Fold change > 4, P < 0.05) were detected in comparisons of TB vs. LTBI and TB vs. HCs, respectively. Nineteen differentially expressed genes with top fold change between TB and the other two groups were validated in the same RNA samples by real-time PCR, and showed 94.7% consistent expression pattern with microarray test.
Project description:Micro RNA profiling was performed on in vitro PPDB and nil stimulated bovine PBMCs isolated from BCG vaccinated and unvaccinated cattle before and after challenge with M. bovis.
Project description:The knowledge of circRNAs in tuberculosis (TB) remains limited. Aim of the present study was to characterize the expression profiles and potential function of circRNAs in human peripheral blood mononuclear cells (PBMCs) from patients with active TB.
Project description:Mycoplasma species are highly contagious pathogens, and Mycoplasma infectious disease are a serious issue for the dairy industry. The bovine peripheral blood mononuclear cells (PBMCs) play an important role for the eradication of pathogens which cause mycoplasmal infection, however the effects of M. bovis for immune response of PBMCs in vitro have not been fully clarified.We examined the transcription profiling of bovine PBMCs on the stimulation with M. bovis for 6h (3 stimuli, 3 control).
Project description:Tuberculosis (TB) is the ninth leading cause of death worldwide and the leading cause from a single infectious agent, ranking above HIV/AIDS.Although considerable advances have been achieved in recent years, the genetic and molecular mechanisms underlying this disease remain largely unclear. In the present research, we used the Affymetrix GeneChip Human Transcriptome Array 2.0 to investgate the profiles of differentially expressed lncRNAs and mRNAs from the peripheral blood mononuclear cells (PBMCs) of 7 TB patients and 5 healthy controls (HC).
Project description:Bovine purified protein derivative (bPPD) and avian purified protein derivative (aPPD) arewidely used forbovine tuberculosis diagnosis. However, little is known about their qualitative and quantitative characteristics, which makes their standardisation difficult. In addition, bPPDcan give false-positive tuberculosis results because of sequence homologybetween Mycobacterium bovis andM. avium proteins. Here we used proteomics to characterisebPPD, aPPD and an immunopurifiedsubcomplexfrombPPD called P22.