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DDM1-facilitated R-loop resolution and H2A.Z exclusion primes heterochromatin formation in Arabidopsis [ssDRIP-seq, ChIP-seq, pNET-seq, ATAC-seq]

ABSTRACT: DDM1-facilitated R-loop resolution and H2A.Z exclusion primes heterochromatin formation in Arabidopsis [ssDRIP-seq, ChIP-seq, pNET-seq, ATAC-seq]

PROVIDER: PRJNA902020 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22306300_1.fastq.gz	Fastqsanger.gz
	SRR22306300_2.fastq.gz	Fastqsanger.gz
	SRR22306301_1.fastq.gz	Fastqsanger.gz
	SRR22306301_2.fastq.gz	Fastqsanger.gz
	SRR22306302_1.fastq.gz	Fastqsanger.gz

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DDM1-facilitated R-loop resolution and H2A.Z exclusion primes heterochromatin formation in Arabidopsis [ssDRIP-seq, ChIP-seq, pNET-seq, ATAC-seq]

Project description:Programmed constitutive heterochromatin silencing is essential for eukaryotic genome regulation, yet the initial step of this process is ambiguous. A large proportion of R-loops (RNA:DNA hybrids) had been unexpectedly identified within Arabidopsis pericentromeric heterochromatin with unknown functions. Through a genome-wide R-loop profiling screen, we find DDM1 (Decrease in DNA Methylation 1) is the primary restrictor of pericentromeric R-loops via its RNA:DNA helicase activity. Low levels of pericentromeric R-loops resolved by DDM1 co-transcriptionally can prime constitutive heterochromatin silencing. Furthermore, we demonstrate that DDM1 physically excludes histone H2A variant H2A.Z, and promotes H2A.W deposition for faithful heterochromatin initiation soon after R-loop clearance. The dual functions of DDM1 in R-loop resolution and H2A.Z eviction are essential for sperm nuclei structure maintenance in mature pollen. Our work unravels the co-transcriptional R-loop resolution coupled with accurate H2A variants deposition is the primary step of constitutive heterochromatin silencing in Arabidopsis, which might be conserved across eukaryotes.

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