Project description:PURPOSE: To provide a detailed gene expression profile of the normal postnatal mouse cornea. METHODS: Serial analysis of gene expression (SAGE) was performed on postnatal day (PN)9 and adult mouse (6 week) total corneas. The expression of selected genes was analyzed by in situ hybridization. RESULTS: A total of 64,272 PN9 and 62,206 adult tags were sequenced. Mouse corneal transcriptomes are composed of at least 19,544 and 18,509 unique mRNAs, respectively. One third of the unique tags were expressed at both stages, whereas a third was identified exclusively in PN9 or adult corneas. Three hundred thirty-four PN9 and 339 adult tags were enriched more than fivefold over other published nonocular libraries. Abundant transcripts were associated with metabolic functions, redox activities, and barrier integrity. Three members of the Ly-6/uPAR family whose functions are unknown in the cornea constitute more than 1% of the total mRNA. Aquaporin 5, epithelial membrane protein and glutathione-S-transferase (GST) omega-1, and GST alpha-4 mRNAs were preferentially expressed in distinct corneal epithelial layers, providing new markers for stratification. More than 200 tags were differentially expressed, of which 25 mediate transcription. CONCLUSIONS: In addition to providing a detailed profile of expressed genes in the PN9 and mature mouse cornea, the present SAGE data demonstrate dynamic changes in gene expression after eye opening and provide new probes for exploring corneal epithelial cell stratification, development, and function and for exploring the intricate relationship between programmed and environmentally induced gene expression in the cornea. Keywords: other

2004-01-02 | GSE887 | GEO

E-GEOD-16364

Project description:Gene expression profile in mouse embryonic stem cells in which endogenous Pcl2 expression was stably knocked down using short hairpin RNA

2015-09-03 | E-GEOD-16364 | ExpressionAtlas

E-GEOD-37859

Project description:Gene expression profile of mouse induced neural stem cells

2015-09-29 | E-GEOD-37859 | ExpressionAtlas

E-GEOD-1294

Project description:Transcription profiling by array of brain from Down syndrome mouse model

2014-07-03 | E-GEOD-1294 | ExpressionAtlas

E-GEOD-30784

Project description:Gene expression profile of OSCC, oral dysplasia, and normal oral tissue

2025-01-24 | E-GEOD-30784 | ExpressionAtlas

Hippocampus proteome Mus musculus

Project description:To describe the protein profile in hippocampus, colon and ileum tissue’ changing after the old faeces transplants, we adopted a quantitative label free proteomics approach.

2021-09-09 | PXD016432 | Pride

E-GEOD-72165

Project description:Expression profile of mouse carotid body and adrenal medulla [RNA-Seq]

2019-05-14 | E-GEOD-72165 | ExpressionAtlas