Ontology highlight
ABSTRACT:
PROVIDER: PRJNA904239 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Plastics are one of the most preoccupying emerging pollutants. Macroplastics released in the environment degrade into microplastics and nanoplastics. Because of their small size, these micro and nano plastic particles can enter the food chain and, in addition to their ecotoxicological effects, contaminate humans with still unknown biological effects. Plastics being particulate pollutants, they are handled in the human body by scavenger cells such as macrophages, which are important players in the immune system. In order to get a better appraisal of the effects of plastic particles on macrophages, we have studied the effects of unmodified polystyrene particles of 0.1, 1 and 10 micrometers of diameter, by a combination of proteomics and targeted approaches. Proteomics showed important adaptive changes of the proteome in response to exposure to plastics, with more than one third of the detected proteins showing a significance change in their abundance in response to cell exposure to at least one plastic beads size. These changes affected for example mitochondrial, lysosomal or cytoskeletal proteins. Although an increase in the mitochondrial transmembrane potential was detected in response to 10 micrometer beads, no alteration in cell viability was observed. Similarly, no lysosomal dysfunction and no alteration in the phagocytic capability of the cells was observed in response to exposure to plastic. When the inflammatory response was examined, no increase in the secretion of tumor necrosis factor or interleukin 6 was observed. Oppositely, the secretion of these cytokines in response to lipopolysaccharide was observed after exposure to plastic, which suggested a decreased ability of macrophages to respond to bacterial infection. In conclusion, these results provide a better understanding of the responses of macrophages to exposure to polystyrene particles of different sizes.
2024-09-09 | PXD031609 | Pride
Project description:The immuno-modulation of exosomes via miRNAs in Zhikong scallop Chlamys farreri
| PRJNA588968 | ENA
Project description:RNA-seq reveals the immuno-modulation of exosomes in Zhikong scallop Chlamys farreri
| PRJNA588477 | ENA
Project description:As marine invertebrates, scallops lack adaptive immunity and employ innate immunity as the front line and almost the solo defense mechanism to protect them against invaders. Accumulating research achievements demonstrated that exosomes could act as innate immune effectors that contribute to host defense mechanism. To better understand the immune functions of exosomes in Chlamys farreri, mRNA profiles of hemocytes from scallops injected with PBS, with normal exosomes and LPS stimulating exosomes, respectively, were generated by deep sequencing, in triplicate.
2022-11-08 | GSE140176 | GEO
Project description:As marine invertebrates, scallops lack adaptive immunity and employ innate immunity as the front line and almost the solo defense mechanism to protect them against invaders. Accumulating research achievements demonstrated that exosomes could act as innate immune effectors that contribute to host defense mechanism. To better understand the immune functions of exosomes in Chlamys farreri, miRNA profiles of hemocytes from scallops injected with PBS, with normal exosomes and LPS stimulating exosomes, respectively, were generated by deep sequencing, in triplicate.
2022-11-12 | GSE140219 | GEO
Project description:To explore the protein components for scallop byssus, the soluble fractions of scallop byssus was extract. For mass spectrometric analysis, proteins were extracted from byssal adhesive plaques, and the whole protein smple was treated with trypsin and analyzed using Thermo Fisher Q Exactive Mass Spectrometer (Thermo Fisher Scientific, USA). The mass spectrometry raw data were searched against the full set of predicted proteins from the C. farreri genome and Transcriptome using Mascot v2.3.0 (Matrix Science, London, UK).
2018-10-23 | PXD007987 | Pride
Project description:To explore the protein components for scallop byssus, the soluble fractions of scallop byssus was extract. For mass spectrometric analysis, proteins were extracted from byssal adhesive plaques, and the major SDS-PAGE fractions was treated with trypsin and analyzed using an Easy-nLC nanoflow HPLC system connected to an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, USA). The mass spectrometry raw data were searched against the full set of predicted proteins from the C. farreri genome using Mascot v2.3.0 (Matrix Science, London, UK).
2018-10-23 | PXD007932 | Pride