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Scallop byssal protein identification

ABSTRACT: To explore the protein components for scallop byssus, the soluble fractions of scallop byssus was extract. For mass spectrometric analysis, proteins were extracted from byssal adhesive plaques, and the major SDS-PAGE fractions was treated with trypsin and analyzed using an Easy-nLC nanoflow HPLC system connected to an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, USA). The mass spectrometry raw data were searched against the full set of predicted proteins from the C. farreri genome using Mascot v2.3.0 (Matrix Science, London, UK).

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Azumapecten Farreri

TISSUE(S): Byssus

SUBMITTER: Xiaokang Zhang

LAB HEAD: Liu Weizhi

PROVIDER: PXD007932 | Pride | 2018-10-23

REPOSITORIES: pride

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			Action	DRS
	P16087-2-2.msf	Msf
	P16087-2-2.pep.pep.xml	Pepxml
	P16087-2-2.prot.prot.xml	Xml
	P16087-2-2.raw	Raw

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Project description:To explore the protein components for scallop byssus, the soluble fractions of scallop byssus was extract. For mass spectrometric analysis, proteins were extracted from byssal adhesive plaques, and the whole protein smple was treated with trypsin and analyzed using Thermo Fisher Q Exactive Mass Spectrometer (Thermo Fisher Scientific, USA). The mass spectrometry raw data were searched against the full set of predicted proteins from the C. farreri genome and Transcriptome using Mascot v2.3.0 (Matrix Science, London, UK).

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