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Age-associated changes in murine endothelial gene expression and chromatin accessibility correlate with impaired blood-brain barrier integrity and elevated risk of intracerebral hemorrhage [ATAC-seq]

ABSTRACT: Age-associated changes in murine endothelial gene expression and chromatin accessibility correlate with impaired blood-brain barrier integrity and elevated risk of intracerebral hemorrhage [ATAC-seq]

PROVIDER: PRJNA904754 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22395773.fastq.gz	Fastqsanger.gz
	SRR22395774.fastq.gz	Fastqsanger.gz
	SRR22395775.fastq.gz	Fastqsanger.gz
	SRR22395776.fastq.gz	Fastqsanger.gz
	SRR22395777.fastq.gz	Fastqsanger.gz

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Age-associated changes in murine endothelial gene expression correlate with impaired blood-brain barrier integrity and elevated risk of intracerebral hemorrhage.

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Examining Transcriptomic Alterations in Rat Models of Intracerebral Hemorrhage and Severe Intracerebral Hemorrhage

Project description:Intracerebral hemorrhage (ICH) is a life-threatening condition associated with significant morbidity and mortality. Understanding the molecular mechanisms underlying ICH and its severe form is crucial for developing effective therapeutic strategies. This study investigates transcriptomic alterations in rodent models of ICH and severe intracerebral hemorrhage to shed light on the genetic pathways involved in hemorrhagic brain injury. We performed principal component analysis, revealing distinct principal component segments of normal rats compared to intracerebral hemorrhage and severe intracerebral hemorrhage rats. We further employed heatmaps and volcano plots to identify differentially expressed genes and utilized bar plots and KEGG pathway analysis to elucidate the different molecular pathways involved. Using comprehensive RNA sequencing and bioinformatics analyses, we identified a multitude of differentially expressed genes in both the ICH and severe ICH models. Our results revealed 5679 common genes among the normal, intracerebral hemorrhage, and severe intracerebral hemorrhage groups in the upregulated genes group, and 1196 common genes in the downregulated genes. A volcano plot comparing the groups further highlighted common genes, including PDPN, TIMP1, SERPINE1, TUBB6, and CD44. These findings underscore the complex interplay of genes involved in inflammation, oxidative stress, and neuronal damage. Furthermore, pathway enrichment analysis uncovered key signaling pathways, including the TNF signaling pathway, protein processing in the endoplasmic reticulum, MAPK signaling pathway, and Fc gamma R-mediated phagocytosis, implicated in the pathogenesis of ICH.

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Age-associated changes in murine endothelial gene expression and chromatin accessibility correlate with impaired blood-brain barrier integrity and elevated risk of intracerebral hemorrhage [RRBS]

Project description:Intracerebral hemorrhage (ICH), a devastating form of stroke, is a leading global cause of human death and disability. The major risk factors for ICH include increasing age, hypertension and cerebral amyloid angiopathy. Despite high mortality and morbidity associated with ICH, the mechanisms leading to blood-brain barrier (BBB) dysfunction with age and development of hemorrhagic stroke is poorly understood. In the vasculature of the central nervous system, endothelial cells (ECs) constitute the core component of the BBB and provide a physical barrier due to tight junctions, adherens junctions, and basement membrane layers. In this study, we show in brains of mice that incidents of intracerebral bleeding increase with advancing age. After isolation of an enriched population of cerebral ECs, we studied gene expression in ECs isolated from murine brains of increasing ages of 2, 6, 12, 18, and 24 months. The study reveals age-dependent dysregulation of 1388 genes in the ECs, including many involved in the maintenance of BBB and vascular integrity. Since epigenetic mechanisms regulate gene expression, we also investigated age-dependent changes at the levels of CpG methylation and accessible chromatin in cerebral ECs. Our study reveals correlations between age-dependent changes in chromatin structure and gene expression. We find significant age-dependent downregulation of the apelin receptor (Aplnr) gene along with an age-dependent reduction in chromatin accessibility of the promoter of this gene. Aplnr is known to play a crucial role in positive regulation of vasodilation and is implicated in vascular health. Interestingly, we also observe an age-dependent reduction in the protein expression levels of the apelin receptor in the brain, potentially implicating the apelin receptor to be critical for the increased risk of intracerebral hemorrhage with ageing.

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Age-associated changes in murine endothelial gene expression and chromatin accessibility correlate with impaired blood-brain barrier integrity and elevated risk of intracerebral hemorrhage [ATAC-seq]

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Age-associated changes in murine endothelial gene expression and chromatin accessibility correlate with impaired blood-brain barrier integrity and elevated risk of intracerebral hemorrhage

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