Ontology highlight
ABSTRACT:
PROVIDER: PRJNA905482 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Over the last 20 years, the advances in sequencing technologies highlighted the unique composition of the salivary glands of blood-feeding arthropods. Further biochemical and structural data demonstrated that salivary proteins can disrupt host hemostasis, inflammation and immune response in addition to favor pathogen transmission. Previously, a Sanger-based sialome of the adult Ochlerotatus. triseriatus female salivary glands was published based on 731 ESTs. Here we revisited O. triseriatus salivary glands contents using an Illumina-sequencing approach of both male and female tissues. In the current data set we report 10,317 coding DNA sequences classified into several functional classes. The translated transcripts also served as reference database for the proteomic analysis of O. triseriatus female saliva, in which unique peptides of 101 proteins were found. Finally, comparison of male and female libraries allowed the identification of female-enriched transcripts that are potentially related to blood acquisition and virus transmission.
2022-10-15 | PXD033588 | Pride
Project description:Mandarin fish (Siniperca chuatsi) has become one of the most commercially important freshwater aquaculture species in China because of its fast growth and high nutritional value. Here, the proteome of the spleen of pathogenetic and resistant mandarin fish on the 8th day after ISKNV infection were analyzed using Illumina NovaSeq 6000 and isobaric tag for relative and absolute quantitation. The spleen tissue of the control group (C), resistant group (K), and pathogenetic group (B) were collected at 8 dpc.
2021-07-09 | PXD027200 |
Project description:To characterize the site-specific methylation landscape of the Mandarin fish ranavirus (MRV) genome, whole-genome bisulfite sequencing (WGBS) was conducted on an isolated MRV strain.
2024-04-02 | GSE262554 | GEO
Project description:Mandarin fish Siniperca chuatsi (Basilewsky) (Percichthyidae), as a demersal piscivore, has very specialized feeding habits, for as soon as they start feeding the fry of this fish feed solely on fry of other fish species. In rearing conditions, mandarin fish has been found to accept live prey fish only, and refuse dead prey fish or artificial diets, very little is currently known about the molecular mechanisms of multiple genes which cover different pathways influencing the specialized food habit, such as live prey. We performed transcriptome comparisons between dead prey fish feeders and nonfeeders in mandarin fish. The determination mechanisms of specialized food habit (live prey fish) in mandarin fish could provide some instructions for research of food habit in animals, including mammals.
2013-10-21 | E-MTAB-1365 | biostudies-arrayexpress
Project description:The discus fish (Symphysodon aequifasciatus) is an ornamental fish, which occupies important position on the freshwater aquarium trade. We built two cDNA libraries from an adult male brain and an adult female brain, and performed RNA-sequencing for identifying sex-biased candidate genes , a total number of 40209 non-redundant genes (unigenes) were obtained, of which 250 unigenes were significant overexpressed in the male brain, and 436 unigenes were significant overexpressed in the female. A total of 439 miRNAs were identified, of which 60 miRNAs were differentially expressed between male brain and female brain. These results can provide important evidence for better understanding the molecular mechanisms of the brain's amphoteric dimorphism in discus fish
2022-11-13 | GSE140349 | GEO
Project description:The discus fish, Symphysodon spp., a South American cichlid, has a unique parental care behavior where fry bite on parental skin mucus after hatching. In this study, we used LC-MS/MS technique to compare the skin mucus proteome composition of male or female discus fish during parental and non-parental care periods. By multivariate statistical analysis, we found clear separations between different periods and between different sexes of mucus proteome. Compared with non-parental female fish, parental female fish had 283 up-regulated and 235 down-regulated expressed proteins. Compared with non-parental male fish, parental male fish had 169 up-regulated and 120 down-regulated expressed proteins. The differentially expressed proteins for male fish were enriched in sulfur relay system, mucin type O-glycan biosynthesis and antigen processing and presentation pathways, while those for female fish were enriched in sulfur relay system, steroid biosynthesis and complement and coagulation cascades pathways. During the parental care, both male and female discus showed an enhanced lipid metabolism, producing more phospholipids and cholesterol. The difference is that male discus had increased tricarboxylic acid cycle producing more energy during the parental care, while females produced more nucleotides especially guanylic acid. Our study could provide new insights into the understanding of the unique mucus supply behavior of discus fish based on proteomic change.
2024-08-15 | PXD054942 |